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Accession: MIC-GCED-1702 Research Theme: Microbiology (Directed Study)
Contributors: James T. Hollibaugh, Qian Liu, Meredith Ross, Jelani Cheek, Corinne Sweeney, Bradley Tolar, Patrick Hagan, Hannah Whitby, Annie Bratcher, Erica Malagon, Nicole Lynn-Bell, Jacob Shalack, Caroline Reddy, John Walker
Title: Coupling between Sediment and Water Column Populations of Ammonia Oxidizing Thaumarchaeota in the Duplin River near Sapelo Island, Georgia
Abstract: Populations of nitrifying organisms in the water column at Marsh Landing display a midsummer peak in the abundance of ammonia oxidizing Archaea (AOA) at the site, coinciding with a peak in nitrite concentration. Marsh Landing is at the mouth of the Duplin River, a dead-end tidal channel that drains an extensive area of salt marsh. While the lower Duplin River at Marsh Landing exchanges tidally with Doboy Sound and thus South Atlantic Bight (SAB) coastal waters, water in its upper reaches has a residence time of weeks. The work reported here had two goals: 1) test the hypothesis that the surrounding salt marsh is the source of nitrifiers seen in water samples taken at Marsh Landing; and 2) compare the seasonal dynamics of nitrifiers in surficial sediments with those in the water column. We sampled 6 stations along the ~20 km length of the Duplin River. We collected surface water samples (~0.20 m) at low- to mid-tide, monthly from April-December 2014. Sediment samples (top 1 cm) were collected at the same time from unvegetated creek bank at 2 locations on the Duplin River and from 4 locations spanning the creek bank-to-upland gradient of the saltmarsh accessible from the Teal Boardwalk. The abundance of ammonia oxidizing Archaea, Marine Group 1 Archaea (Thaumarchaeota), ammonia oxidizing Betaproteobacteria (AOB), Bacteria and Nitrospina, a nitrite oxidizing bacterium, were determined by quantitative PCR (qPCR) of DNA extracted from the samples. This data set contains the abundance estimates from April to December 2014 for sediment and water column samples, with corresponding water quality measurements (temperature, salinity and nitrogenous nutrient concentrations).
DOI: 10.6073/pasta/cc05fd510d50999d73d903cf10e308df
Key Words: ammonia, ammonia oxidizing archaea, Archaea, estuaries, microbes, nitrate, nitrite, nitrogen compounds, salinity, salt marshes, sediments, temperature, Thaumarchaeota, urea, water
LTER Core Area: Other Site Research
Research Themes: GCE3 Area2 - Patterns within the Domain, Microbiology, General Nutrient Chemistry
Study Period: 09-Apr-2014 to 01-Dec-2014
Study Sites:
GCE10 -- Hunt Camp, Sapelo Island, Georgia, USA
ML -- Marsh Landing, Sapelo Island, Georgia, USA
» Download Geographic Coverage: Google Earth, ESRI Shapefile (polygons, points)
Project References: Ammonia oxidation in the GCE domain
Publications:

Hollibaugh, J.T., Liu, Q., Ross, M., Cheek, J., Sweeney, C., Tolar, B., Hagan, P., Whitby, H., Bratcher, A., Malagon, E., Lynn-Bell, N., Shalack, J., Reddy, C.M. and Walker, J.T. (in review). Coupling between Sediment and Water Column Populations of Ammonia Oxidizing Thaumarchaeota in a Salt Marsh Estuary.

Downloads: Information

Data Table: MIC-GCED-1702_Sediment (Sediment analyses for data set MIC-GCED-1702, 124 records)

Access: Public (released 10-Feb-2018)

Metadata: Text (ESA FLED), XML (Ecological Metadata Language)

Data Formats: Spreadsheet (CSV) [13.45kb],  Text File [11.37kb],  Text Report [35.65kb],  MATLAB (GCE Toolbox) [222.37kb],  MATLAB (Variables) [132.80kb]

Column List:(display)


Data Table: MIC-GCED-1702_Water (Water column analyses for data set MIC-GCED-1702, 150 records)

Access: Public (released 10-Feb-2018)

Metadata: Text (ESA FLED), XML (Ecological Metadata Language)

Data Formats: Spreadsheet (CSV) [33.01kb],  Text File [28.80kb],  Text Report [57.30kb],  MATLAB (GCE Toolbox) [274.23kb],  MATLAB (Variables) [184.71kb]

Column List:(display)

Statistics: Generate script code to retrieve data tables for analysis in: MATLAB, R, SAS, SPSS
Citation: Hollibaugh, James T. 2017. Coupling between Sediment and Water Column Populations of Ammonia Oxidizing Thaumarchaeota in the Duplin River near Sapelo Island, Georgia. Georgia Coastal Ecosystems LTER Project, University of Georgia, Long Term Ecological Research Network. http://dx.doi.org/10.6073/pasta/cc05fd510d50999d73d903cf10e308df

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This material is based upon work supported by the National Science Foundation under grants OCE-9982133, OCE-0620959 and OCE-1237140. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.