Ammonia oxidation in the GCE domain Dr. James T. Hollibaugh University of Georgia

Dept. of Marine Sciences University of Georgia Athens Georgia 30602-3636 USA

aquadoc@uga.edu http://www.marsci.uga.edu/directory/james-t-hollibaugh https://orcid.org/0000-0001-8037-160X Georgia Coastal Ecosystems LTER Project

Dept. of Marine Sciences University of Georgia Athens Georgia 30602-3636 USA

gcelter@uga.edu https://gce-lter.marsci.uga.edu/ Dr. James T. Hollibaugh University of Georgia

Dept. of Marine Sciences University of Georgia Athens Georgia 30602-3636 USA

aquadoc@uga.edu http://www.marsci.uga.edu/directory/james-t-hollibaugh https://orcid.org/0000-0001-8037-160X Principal investigator Dr. Jane M. Caffrey University of West Florida

Center for Diagnostics and Bioremediation University of West Florida Pensacola Florida 32514 USA

jcaffrey@uwf.edu http://uwf.edu/jcaffrey/ Co-investigator Patrick Hagan Sapelo Island National Estuarine Research Reserve

Sapelo Island National Estuarine Research Reserve P.O. Box 15 Sapelo Island Georgia 31327 USA

bluewatermotion@hotmail.com Co-investigator 2026

The goal of this project is to determine the seasonal variation in ammonia oxidizer abundance in the Duplin River. We collect samples from the dock at Marsh Landing, take them to the lab, filter them, freeze the filter, use qPCR to measure abundance. We also collect samples for DIN analyses.

Area 2: Patterns within the Domain (more information)

water column/neritic nutrients LTER NSF GCE archaea microbes/fungi microbiology nutrient chemistry Overall geographic extent of the research project -81.282950 -81.278834 31.398877 31.395295 2004-04-15

NSF OCE 03-52216

estuary marsh complex UGA Marine Institute - The University of Georgia Marine Institute is located on the southern end of Sapelo Island, a barrier island off the central Georgia coast. -81.282950 -81.278834 31.398877 31.395295 Map (Google Earth KML/KMZ or map image) https://gce-lter.marsci.uga.edu/private/registration/files/maps/GCE-22-2012_Maps_Hollibaugh_ammonia_oxidizer_study_2012.kml

UGAMI, UGA

Protozoa and phytoplankton samples are collected for analysis.

Liu, Q., Tolar, B., Ross, M., Cheek, J., Sweeney, C., Wallsgrove, N.J., Popp, B.N. and Hollibaugh, J.T. 2018. Light and temperature control the seasonal distribution of Thaumarchaeota in the South Atlantic Bight. ISME Journal. 12:1473-1485. (DOI: 10.1038/s41396-018-0066-4) Damashek, J., Tolar, B., Liu, Q., Okotie-Oyekan, A., Wallsgrove, N.J., Popp, B.N. and Hollibaugh, J.T. 2018. Microbial oxidation of nitrogen supplied as selected organic nitrogen compounds in the South Atlantic Bight. Limnology and Oceanography. 64:982-995. (DOI: 10.1002/lno.11089) Hollibaugh, J.T., Bratcher, A., Cheek, J., Liu, Q., Malagon, E., Popp, B.N., Ross, M., Schaefer, S.C., Sweeney, C., Tolar, B., van den Berg, C.M., Wallsgrove, N.J. and Whitby, H. 2017. Poster: LIGHT AND TEMPERATURE CONTROL THE SEASONAL DISTRIBUTION OF THAUMARCHAEOTA IN THE SOUTH ATLANTIC BIGHT. Fifth International Conference on Nitrification and Related Processes (ICoN5)23-27 July, 2017, 23-27 July, 2017, Vienna, Austria. http://gce-lter.marsci.uga.edu/public/uploads/ICoN-5_Abstract_20170719T185828.docx Whitby, H., Hollibaugh, J.T. and van den Berg, C.M. 2017. Chemical speciation of copper in a salt marsh estuary and bioavailability to Thaumarchaeota. Special Issue: Organic ligands - A key control on trace metal biogeochemistry in the ocean. Frontiers in Marine Sciences. 4. (DOI: 10.3389/fmars.2017.00178) http://journal.frontiersin.org/article/10.3389/fmars.2017.00178/full Hollibaugh, J.T., Liu, Q., Ross, M., Cheek, J., Sweeney, C., Tolar, B., Hagan, P., Whitby, H., Bratcher, A., Malagon, E., Lynn-Bell, N., Shalack, J., Reddy, C.M. and Walker, J.T. 2016. Poster: Coupling between Sediment and Water Column Populations of Ammonia Oxidizing Thaumarchaeota in a Salt Marsh Estuary. Tolar, B., Wallsgrove, N.J., Popp, B.N. and Hollibaugh, J.T. 2016. Oxidation of urea nitrogen in marine nitrifying communities dominated by Thaumarchaeota. Environmental Microbiology. (DOI: 10.1111/1462-2920.13457) http://gce-lter.marsci.uga.edu/public/uploads/Tolar_et_al_2016_Environ_Microbiol_20160906T155450.pdf Caffrey, J.M., Hollibaugh, J.T. and Mortazavi, B. 2016. Living oysters and their shells as sites of nitrification and denitrification. Marine Pollution Bulletin. (DOI: 10.1016/j.marpolbul.2016.08.038.) http://gce-lter.marsci.uga.edu/public/uploads/Caffrey_et_al_2016_20160906T154700.pdf CHM-OTH-1702 - Chemical speciation of copper in a salt marsh estuary near Sapelo Island, Georgia https://gce-lter.marsci.uga.edu/public/app/dataset_details.asp?id=612 MIC-GCED-1702 - Coupling between Sediment and Water Column Populations of Ammonia Oxidizing Thaumarchaeota in the Duplin River near Sapelo Island, Georgia https://gce-lter.marsci.uga.edu/public/app/dataset_details.asp?id=618 MIC-GCED-1704 - Seasonal Distribution of Ammonia-Oxidizing Archaea and Ammonia-Oxidation Rates in the South Atlantic Bight from April to November 2014 https://gce-lter.marsci.uga.edu/public/app/dataset_details.asp?id=620