I. Data Set Descriptors A. Title: Robert E. Hodson. 2003. November 2001 surface water bacterial productivity at ten Georgia Coastal Ecosystems LTER sampling sites. Georgia Coastal Ecosystems LTER Data Catalog (data set BCT-GCEM-0302c; http://gce-lter.marsci.uga.edu/public/app/dataset_details.asp?accession=BCT-GCEM-0302c) B. Accession Number: BCT-GCEM-0302c C. Description 1. Originator(s): Name: Robert E. Hodson Address: Dept. of Marine Sciences University of Georgia Athens, Georgia 30602-3636 Country: USA Email: rhodson@uga.edu 2. Abstract: Surface water samples were collected during low tide survays near ten Georgia Coastal Ecosystem LTER sampling sites in November, 2001. The incorporation of tritiated leucine in unfiltered samples during one hour incubations was measured using a standard microcentrifuge method to estimate bacterial productivity in each sample. This study was part of the GCE-LTER hydrographic monitoring program, and will be repeated quarterly. 3. Study Type: Monitoring 4. Study Themes: Bacterial Productivity 5. LTER Core Areas: Populations 6. Georeferences: geographic coordinates as data columns 7. Submission Date: Feb 25, 2003 D. Keywords: bacteria, bacterial production, leucine, microbial, Microbial Populations, Populations, water column II. Research Origin Descriptors A. Overall Project Description 1. Project Title: Georgia Coastal Ecosystems LTER Project 2. Principal Investigators: Name: James T. Hollibaugh Address: Dept. of Marine Sciences University of Georgia Athens, Georgia 30602-3636 Country: USA Email: aquadoc@uga.edu 3. Funding Period: May 01, 2000 to May 01, 2006 4. Objectives: To investigate the linkages between coastal and distant upland areas in central Georgia mediated by surface and ground water delivery to the coastal zone. This will be addressed by examining the relationship between variability in environmental factors driven by river flow, primarily salinity, and ecosystem processes and structure. 5. Abstract: We propose to establish a Long Term Ecological Research site on the central Georgia coast in the vicinity of Sapelo Island. This is a barrier island and marsh complex with the Altamaha River, one of the largest and least developed rivers on the east coast of the US, as the primary source of fresh water. The proposed study would investigate the linkages between local and distant upland areas mediated by water - surface water and ground water - delivery to the coastal zone. We would explicitly examine the relationship between variability in environmental factors driven by river flow, primarily salinity because we can measure it at high frequency, and ecosystem processes and structure. We will accomplish this by comparing estuary/marsh complexes separated from the Altamaha River by one or two lagoonal estuary/marsh complexes that damp and attenuate the river signal. This spatial gradient is analogous to the temporal trend in riverine influence expected as a result of development in the watershed. We will implement a monitoring system that documents physical and biological variables and use the time trends and spatial distributions of these variables and of their variance structure to address questions about the factors controlling distributions, trophic structure, diversity, and biogeochemistry. An existing GIS-based hydrologic model will be modified to incorporate changes in river water resulting from changes in land use patterns that can be expected as the watershed develops. This model will be linked to ecosystem models and will serve as an heuristic and management tool. Another consequence of coastal development is that as river flow decreases, groundwater flow increases and becomes nutrified. We will compare the effects of ground water discharge from the surficial aquifer in relatively pristine (Sapelo Island) versus more urbanized (mainland) sites to assess the relative importance of fresh water versus nutrients to productivity, structure and biomass turnover rate in marshes influenced by groundwater. We will also investigate the effect of marine processes (tides, storm surge) on mixing across the fresh/salt interface in the surficial aquifer. Additional physical studies will relate the morphology of salt marsh - tidal creek channel complexes to tidal current distributions and exchange. These findings will be incorporated into a physical model that will be coupled to an existing ecosystem model. The land/ocean margin ecosystem lies at the interface between two ecosystems in which distinctly different groups of decomposers control organic matter degradation. The terrestrial ecosystem is largely dominated by fungal decomposers, while bacterial decomposers dominate the marine ecosystem. Both groups are important in salt marsh-dominated ecosystems. Specific studies will examine, at the level of individual cells and hyphae, the relationship bacteria and fungi in the consortia that decompose standing dead Spartina and other marsh plants and examine how, or if, this changes along the salinity gradient. 6. Funding Source: NSF OCE 9982133 B. Sub-project Description 1. Site Description a. Geographic Location: GCE1 -- Eulonia, Georgia, USA GCE2 -- Four Mile Island, Georgia, USA GCE3 -- North Sapelo, Sapelo Island, Georgia, USA GCE4 -- Meridian, Georgia, USA GCE5 -- Folly River, Georgia, USA GCE6 -- Dean Creek, Sapelo Island, Georgia, USA GCE7 -- Carrs Island, Georgia, USA GCE8 -- Alligator Creek, Georgia, USA GCE9 -- Rockdedundy Island, Georgia, USA GCE10 -- Hunt Camp, Sapelo Island, Georgia, USA GCE-SP -- Sapelo River, Georgia, USA GCE-DB -- Doboy Sound, Georgia, USA GCE-AL -- Altamaha River, Georgia, USA GCE-DP -- Duplin River, Georgia, USA AL+02 -- AL+02 AL+14 -- AL+14 AL+24 -- AL+24 DB+00 -- DB+00 DB+08 -- DB+08 DB+12.6 -- DB+12.6 DUP5 -- Duplin River station 5 (Hunt Camp) SP+03 -- SP+03 SP+12 -- SP+12 SP+36 -- SP+36 Coordinates: GCE1 -- NW: 081 25 42.53 W, 31 32 48.30 N NE: 081 24 38.64 W, 31 32 48.30 N SE: 081 24 38.64 W, 31 32 02.27 N SW: 081 25 42.53 W, 31 32 02.27 N GCE2 -- NW: 081 19 08.26 W, 31 33 09.19 N NE: 081 17 34.24 W, 31 33 09.19 N SE: 081 17 34.24 W, 31 32 00.97 N SW: 081 19 08.26 W, 31 32 00.97 N GCE3 -- NW: 081 13 59.70 W, 31 32 48.72 N NE: 081 11 44.38 W, 31 32 48.72 N SE: 081 11 44.38 W, 31 30 54.68 N SW: 081 13 59.70 W, 31 30 54.68 N GCE4 -- NW: 081 22 14.71 W, 31 27 48.72 N NE: 081 21 26.12 W, 31 27 48.72 N SE: 081 21 26.12 W, 31 26 54.31 N SW: 081 22 14.71 W, 31 26 54.31 N GCE5 -- NW: 081 21 15.89 W, 31 26 33.14 N NE: 081 19 37.92 W, 31 26 33.14 N SE: 081 19 37.92 W, 31 25 03.50 N SW: 081 21 15.89 W, 31 25 03.50 N GCE6 -- NW: 081 17 58.28 W, 31 23 38.66 N NE: 081 15 51.76 W, 31 23 38.66 N SE: 081 15 51.76 W, 31 22 15.61 N SW: 081 17 58.28 W, 31 22 15.61 N GCE7 -- NW: 081 29 36.59 W, 31 20 55.59 N NE: 081 28 33.24 W, 31 20 55.59 N SE: 081 28 33.24 W, 31 20 03.71 N SW: 081 29 36.59 W, 31 20 03.71 N GCE8 -- NW: 081 25 34.58 W, 31 19 04.11 N NE: 081 24 38.28 W, 31 19 04.11 N SE: 081 24 38.28 W, 31 18 14.63 N SW: 081 25 34.58 W, 31 18 14.63 N GCE9 -- NW: 081 20 48.23 W, 31 21 29.31 N NE: 081 19 35.38 W, 31 21 29.31 N SE: 081 19 35.38 W, 31 20 21.02 N SW: 081 20 48.23 W, 31 20 21.02 N GCE10 -- NW: 081 17 43.82 W, 31 29 49.29 N NE: 081 15 32.07 W, 31 29 49.29 N SE: 081 15 32.07 W, 31 27 44.35 N SW: 081 17 43.82 W, 31 27 44.35 N GCE-SP -- NW: 081 25 55.13 W, 31 33 34.66 N NE: 081 09 14.84 W, 31 33 34.66 N SE: 081 09 14.84 W, 31 30 59.89 N SW: 081 25 55.13 W, 31 30 59.89 N GCE-DB -- NW: 081 22 05.66 W, 31 27 20.39 N NE: 081 14 34.64 W, 31 27 20.39 N SE: 081 14 34.64 W, 31 20 27.39 N SW: 081 22 05.66 W, 31 20 27.39 N GCE-AL -- NW: 081 34 56.32 W, 31 24 05.05 N NE: 081 14 16.57 W, 31 24 05.05 N SE: 081 14 16.57 W, 31 17 45.72 N SW: 081 34 56.32 W, 31 17 45.72 N GCE-DP -- NW: 081 18 07.15 W, 31 29 11.37 N NE: 081 15 47.25 W, 31 29 11.37 N SE: 081 15 47.25 W, 31 24 39.08 N SW: 081 18 07.15 W, 31 24 39.08 N AL+02 -- 081 18 24.0 W, 31 19 15.0 N AL+14 -- 081 24 54.0 W, 31 18 24.0 N AL+24 -- 081 28 30.0 W, 31 20 12.0 N DB+00 -- 081 16 54.2 W, 31 22 34.9 N DB+08 -- 081 19 46.4 W, 31 25 56.6 N DB+12.6 -- 081 21 42.5 W, 31 27 12.4 N DUP5 -- 081 16 25.0 W, 31 28 46.0 N SP+03 -- 081 12 28.3 W, 31 32 33.5 N SP+12 -- 081 17 57.6 W, 31 32 03.8 N SP+36 -- 081 25 24.6 W, 31 32 12.9 N b. Physiographic Region: GCE1 -- Lower coastal plain GCE2 -- Barrier island GCE3 -- Barrier island GCE4 -- Lower coastal plain GCE5 -- Barrier island GCE6 -- Barrier island GCE7 -- Lower coastal plain GCE8 -- Lower coastal plain GCE9 -- Barrier island GCE10 -- Barrier island GCE-SP -- Estuary and tidal marsh GCE-DB -- Estuary and tidal marsh GCE-AL -- Estuary and tidal marsh GCE-DP -- Myxohaline tidal creek and marsh AL+02 -- Estuary and tidal marsh AL+14 -- Estuary and tidal marsh AL+24 -- Estuary and tidal marsh DB+00 -- Estuary and tidal marsh DB+08 -- Estuary and tidal marsh DB+12.6 -- Estuary and tidal marsh DUP5 -- Myxohaline tidal creek and marsh SP+03 -- Estuary and tidal marsh SP+12 -- Estuary and tidal marsh SP+36 -- Estuary and tidal marsh c. Landform Components: GCE1 -- Intertidal salt marsh bordering maritime forest GCE2 -- Intertidal salt marsh GCE3 -- Intertidal salt marsh, mud flat, and maritime forest GCE4 -- Intertidal salt marsh bordering maritime forest GCE5 -- Intertidal salt marsh GCE6 -- Intertidal salt marsh, sand beach, maritime forest GCE7 -- Freshwater tidal marsh, devegetated fields GCE8 -- Brackish tidal marsh GCE9 -- Intertidal salt marsh GCE10 -- Intertidal salt marsh bordering maritime forest GCE-SP -- Intertidal marsh bordering sounds, tidal channels and creeks GCE-DB -- Intertidal marsh bordering sounds, tidal channels and creeks GCE-AL -- Intertidal marsh bordering sounds, tidal channels and creeks GCE-DP -- Intertidal marsh bordering tidal channels and creeks AL+02 -- Intertidal marsh bordering sounds, tidal channels and creeks AL+14 -- Intertidal marsh bordering sounds, tidal channels and creeks AL+24 -- Intertidal marsh bordering sounds, tidal channels and creeks DB+00 -- Intertidal marsh bordering sounds, tidal channels and creeks DB+08 -- Intertidal marsh bordering sounds, tidal channels and creeks DB+12.6 -- Intertidal marsh bordering sounds, tidal channels and creeks DUP5 -- Intertidal marsh bordering tidal channels and creeks SP+03 -- Intertidal marsh bordering sounds, tidal channels and creeks SP+12 -- Intertidal marsh bordering sounds, tidal channels and creeks SP+36 -- Intertidal marsh bordering sounds, tidal channels and creeks d. Hydrographic Characteristics: GCE1 -- Site contains the upper reaches of the Sapelo River, and is subject to 2-3m semi-diurnal tides GCE2 -- Site contains the Sapelo River and associated tidal creeks, and is subject to 2-3m semi-diurnal tides GCE3 -- Site includes Sapelo Sound and portions of Blackbeard Creek, and is subject to 2-3m semi-diurnal tides GCE4 -- Site includes Hudson Creek and is subject to 2-3m semi-diurnal tides GCE5 -- Site contains the Folly River and borders Doboy Sound, and is subject to 2-3m semi-diurnal tides GCE6 -- Site contains Dean Creek and borders Doboy Sound, and is subject to 2-3m semi-diurnal tides GCE7 -- Site contains Hammersmith Creek and borders the South Champney River, and is subject to 2-3m semi-diurnal tides GCE8 -- Site contains the Altamaha River, and is subject to 2-3m semi-diurnal tides GCE9 -- Site contains Crooked Creek and borders the Little Mud River, and is subject to 2-3m semi-diurnal tides GCE10 -- Site borders the Mud River and contains tidal creeks and the upper reach of the Duplin River, and is subject to 2-3m semi-diurnal tides GCE-SP -- Site includes the Sapelo River and Sapelo Sound, and is subject to 2-3.4m semi-diurnal tides. Sapelo Sound is a lagunal estuary system, with minimal freshwater stream or river input. GCE-DB -- Site encompasses Doboy Sound and nearby tidal channels and creeks, and is subject to 2-3.4m semi-diurnal tides. GCE-AL -- Site encompasses the Altamaha River estuary and nearby tidal creeks, and is subject to 2-3.4m semi-diurnal tides. High freshwater input from the Altamaha River, one of the largest rivers in the Southeastern United States. GCE-DP -- Site includes the Duplin River and various smaller tidal creeks and channels AL+02 -- Site encompasses the Altamaha River estuary and nearby tidal creeks, and is subject to 2-3.4m semi-diurnal tides. High freshwater input from the Altamaha River, one of the largest rivers in the Southeastern United States. AL+14 -- Site encompasses the Altamaha River estuary and nearby tidal creeks, and is subject to 2-3.4m semi-diurnal tides. High freshwater input from the Altamaha River, one of the largest rivers in the Southeastern United States. AL+24 -- Site encompasses the Altamaha River estuary and nearby tidal creeks, and is subject to 2-3.4m semi-diurnal tides. High freshwater input from the Altamaha River, one of the largest rivers in the Southeastern United States. DB+00 -- Site encompasses Doboy Sound and nearby tidal channels and creeks, and is subject to 2-3.4m semi-diurnal tides. DB+08 -- Site encompasses Doboy Sound and nearby tidal channels and creeks, and is subject to 2-3.4m semi-diurnal tides. DB+12.6 -- Site encompasses Doboy Sound and nearby tidal channels and creeks, and is subject to 2-3.4m semi-diurnal tides. DUP5 -- Site includes the Duplin River and various smaller tidal creeks and channels SP+03 -- Site includes the Sapelo River and Sapelo Sound, and is subject to 2-3.4m semi-diurnal tides. Sapelo Sound is a lagunal estuary system, with minimal freshwater stream or river input. SP+12 -- Site includes the Sapelo River and Sapelo Sound, and is subject to 2-3.4m semi-diurnal tides. Sapelo Sound is a lagunal estuary system, with minimal freshwater stream or river input. SP+36 -- Site includes the Sapelo River and Sapelo Sound, and is subject to 2-3.4m semi-diurnal tides. Sapelo Sound is a lagunal estuary system, with minimal freshwater stream or river input. e. Topographic Attributes: GCE1 -- Flat, with elevations ranging from 0-3m above mean low tide GCE2 -- Flat, with elevations ranging from 0-3m above mean low tide GCE3 -- Flat, with elevations ranging from 0-3m above mean low tide GCE4 -- Flat, with elevations ranging from 0-3m above mean low tide GCE5 -- Flat, with elevations ranging from 0-3m above mean low tide GCE6 -- Flat, with elevations ranging from 0-3m above mean low tide GCE7 -- Flat, with elevations ranging from 0-3m above mean low tide GCE8 -- Flat, with elevations ranging from 0-3m above mean low tide GCE9 -- Flat, with elevations ranging from 0-3m above mean low tide GCE10 -- Flat, with elevations ranging from 0-3m above mean low tide GCE-SP -- Flat, with elevations ranging from 0-3.4m above mean low tide GCE-DB -- Flat, with elevations ranging from 0-3.4m above mean low tide GCE-AL -- Flat, with elevations ranging from 0-3.4m above mean low tide GCE-DP -- Flat, with elevations ranging from 0-3.4m above mean low tide AL+02 -- Flat, with elevations ranging from 0-3.4m above mean low tide AL+14 -- Flat, with elevations ranging from 0-3.4m above mean low tide AL+24 -- Flat, with elevations ranging from 0-3.4m above mean low tide DB+00 -- Flat, with elevations ranging from 0-3.4m above mean low tide DB+08 -- Flat, with elevations ranging from 0-3.4m above mean low tide DB+12.6 -- Flat, with elevations ranging from 0-3.4m above mean low tide DUP5 -- Flat, with elevations ranging from 0-3.4m above mean low tide SP+03 -- Flat, with elevations ranging from 0-3.4m above mean low tide SP+12 -- Flat, with elevations ranging from 0-3.4m above mean low tide SP+36 -- Flat, with elevations ranging from 0-3.4m above mean low tide f. Geology, Lithology and Soils: GCE1 -- unspecified GCE2 -- unspecified GCE3 -- unspecified GCE4 -- unspecified GCE5 -- unspecified GCE6 -- unspecified GCE7 -- unspecified GCE8 -- unspecified GCE9 -- unspecified GCE10 -- unspecified GCE-SP -- unspecified GCE-DB -- unspecified GCE-AL -- unspecified GCE-DP -- unspecified AL+02 -- unspecified AL+14 -- unspecified AL+24 -- unspecified DB+00 -- unspecified DB+08 -- unspecified DB+12.6 -- unspecified DUP5 -- unspecified SP+03 -- unspecified SP+12 -- unspecified SP+36 -- unspecified g. Vegetation Communities: GCE1 -- Vegetation is 3/4 Juncus and 1/4 Spartina alterniflora, and upland area is heavily forested. GCE2 -- Spartina alterniflora all tall or medium. GCE3 -- Short and tall Spartina alterniflora, very small amounts of Juncus. GCE4 -- Vegetation is 1/4 Juncus, 3/4 medium and tall Spartina alterniflora. GCE5 -- Dominated by short and medium Spartina alterniflora, with areas of taller S. alterniflora near several small creeks. GCE6 -- Vegetation is mostly short and tall Spartina alterniflora, with small amounts of Juncus and Borrichia. GCE7 -- dominated by Zizaniopsis GCE8 -- Fringe of tall Spartina alterniflora and S. cynosuroides at creek banks. High marsh has narrow band of medium height S. alterniflora backed by vast expanses of Juncus. GCE9 -- Low marsh is tall Spartina alterniflora, high marsh has a small amount of medium Spartina alterniflora and lots of Juncus. Some hammocks with upland vegetation. GCE10 -- Vegetation mostly tall and medium Spartina alterniflora, with some Juncus present. Upland heavily forested. GCE-SP -- unspecified GCE-DB -- unspecified GCE-AL -- unspecified GCE-DP -- unspecified AL+02 -- unspecified AL+14 -- unspecified AL+24 -- unspecified DB+00 -- unspecified DB+08 -- unspecified DB+12.6 -- unspecified DUP5 -- unspecified SP+03 -- unspecified SP+12 -- unspecified SP+36 -- unspecified h. History of Land Use and Disturbance: none recorded i. Climate: Climate summary for Sapelo Island, Georgia, based on NWS data from 1980-2010: Daily-aggregated Values: Mean (sample standard deviation) mean air temperature: 20.09°C (7.28°C) minimum air temperature: 15.02°C (7.96°C) maximum air temperature: 24.82°C (6.98°C) total precipitation: 3.26mm (10.3mm) Yearly-aggregated Daily Values: Mean (sample standard deviation) total precipitation (1980-2010): 1124mm (266mm) 2. Experimental or Sampling Design a. Design Characteristics: Surface water samples were collected at fixed locations near 10 GCE-LTER long-term monitoring sites to examine bacterial productivity. b. Permanent Plots: none c. Data Collection Duration and Frequency: Water samples were collected from the surface during low tidal conditions at fixed locations in areas designated as long-term monitoring sites by the Georgia Coastal Ecosystems LTER project. Beginning of Observations: Nov 26, 2001 End of Observations: Nov 30, 2001 3. Research Methods a. Field and Laboratory Methods: Method 1: Water Collection -- Water samples were collected from the R/V Savannah approximately 1 meter below the surface using a Niskin bottle sampler deployed using a hydrographic winch. The water sample was then transferred to brown 1 L Nalgene bottles, which were pre-rinsed once with approximately 100 ml of sample water prier to filling. Surface samples collected from the R/V Salty Dawg and Carolina Skiff were collected by hand approximately 0.5 meters below the surface of the water using a brown 1 L Nalgene bottle rinsed once with sample water. Method 2: Sample Incubation -- Four sterile 2 ml centrifuge tubes were individually numbered and 5µL of leucine stock solution (20 nM final concentration) was added to each tube [L-(4-5) 3H-Leucine Amersham corparation cat# TRK510, ca. 150mCi/mmol]. Tricarboxylic Acid (TCA) was added to the first tube in each series (89µl of 100% TCA, final concentration of 5%) to function as a killed control. 1.7ml of sample was added into each tube, and the tubes were vortexed and incubated at ambient tempertures for one hour. At the end of the incubation period live samples were fixed with 89 ul 100% TCA and vortexed. Samples were then stored refrigerated and transported to the laboratory for processing. (reference: Smith, D.C., Azam, F., 1992. A simple, economical method for measuring bacterial protein synthesis rates in seawater using 3H-leucine. Marine Microbial Food Webs 6:107-114.) Method 3: Leucine Incorporation Measurement -- Samples were warmed to room temperture and spun for 10 minutes in a bench-top centrifuge at top speed. The supernatant was removed by vacuum aspiration, taking care not to disturb the pellet. The pellet was then resuspended with 1.5ml of 5% TCA using a vortex mixer, and the centrifugation and aspiration step was repeated to rinse the precipitate. 1.5ml of biodegradable scintillation fluid was then added directly into the tube and the suspension was mixed by vortexing. Radioactivity in each tube was measured by liquid scintillation counting (LSC), and disintegrations per minute were calculated from counts per minute by the scintillation counter software based on calculated count efficiency for the samples. (reference: Smith, D.C., Azam, F., 1992. A simple, economical method for measuring bacterial protein synthesis rates in seawater using 3H-leucine. Marine Microbial Food Webs 6:107-114.) b. Instrumentation: Method 1: none Method 2: none Method 3: Liquid Scintillation Counter Manufacturer: Beckman (Model: LS 6500) Parameter: DPM (disintegrations per minute) c. Taxonomy and Systematics: Method 1: not applicable Method 2: not applicable Method 3: not applicable d. Permit History: Method 1: none Method 2: none Method 3: none 4. Project Personnel a. Personnel: 1: Robert E. Hodson 2: Xiaozhen Mou 3: Edward Sheppard b. Affiliations: 1: University of Georgia, Athens, Georgia 2: University of Georgia, Athens, Georgia 3: University of Georgia, Athens, Georgia III. Data Set Status and Accessibility A. Status 1. Latest Update: 20-Jun-2013 2. Latest Archive Date: 18-Nov-2011 3. Latest Metadata Update: 20-Jun-2013 4. Data Verification Status: Reviewed by GCE-LTER IM B. Accessibility 1. Storage Location and Medium: Stored at GCE-LTER Data Management Office Dept. of Marine Sciences Univ. of Georgia Athens, GA 30602-3636 USA on media: electronic data download (WWW) or compact disk 2. Contact Person: Name: Wade M. Sheldon, Jr. Address: Dept. of Marine Sciences University of Georgia Athens, Georgia 30602-3636 Country: USA Email: sheldon@uga.edu 3. Copyright Restrictions: not copyrighted 4. Restrictions: All publications based on this data set must cite the contributor and Georgia Coastal Ecosystems LTER project, and two copies of the manuscript must be submitted to the GCE-LTER Information Management Office. a. Release Date: Affiliates: Feb 25, 2003, Public: Feb 23, 2003 b. Citation: Data provided by the Georgia Coastal Ecosystems Long Term Ecological Research Project, supported by funds from NSF OCE 9982133 (data set BCT-GCEM-0302c) c. Disclaimer: The user assumes all responsibility for errors in judgement based on interpretation of data and analyses presented in this data set. 5. Costs: free electronic data download via WWW, distribution on CD may be subject to nominal processing and handling fee IV. Data Structural Descriptors A. Data Set File 1. File Name: BCT-GCEM-0302c_4_0.CSV 2. Size: 30 records 3. File Format: ASCII text (comma-separated value format) 3a. Delimiters: single comma 4. Header Information: 5 lines of ASCII text 5. Alphanumeric Attributes: mixed case 6. Quality Control Flag Codes: I = invalid value (out of range), Q = questionable value 7. Authentication Procedures: not specified 8. Calculations: Leu_Uptake: Leu_Uptake(pmol/L/hr) = ((DPM_Sample-DPM_Control) / Volume_Sample(ml) / 2.22e6 DPM/µCi / Specific_Activity(µCi/µmol) * 1e6 pmol/µmol) / Time_Incubation(hr) 9. Processing History: Software version: GCE Data Toolbox Version 3.6.0 (05-Jun-2013) Data structure version: GCE Data Structure 1.1 (29-Mar-2001) Original data file processed: BCT-GCEM-0302c.TXT (30 records) Data processing history: 19-May-2003: new GCE Data Structure 1.1 created ('newstruct') 19-May-2003: 30 rows imported from ASCII data file 'BCT-GCEM-0302c.TXT' ('imp_ascii') 19-May-2003: 10 metadata fields in file header parsed ('parse_header') 19-May-2003: data structure validated ('gce_valid') 19-May-2003: Q/C flagging criteria applied, 'flags' field updated ('dataflag') 19-May-2003: imported Data, Dataset, Project, Site, Study, Status, Supplement metadata descriptors from the GCE Metabase ('imp_gcemetadata') 19-May-2003: Q/C flagging criteria applied, 'flags' field updated ('dataflag') 21-May-2003: manually edited data set metadata ('ui_editmetadata') 29-Oct-2003: imported Data, Dataset, Project, Site, Study, Status, Supplement metadata descriptors from the GCE Metabase ('imp_gcemetadata') 29-Oct-2003: Q/C flagging criteria applied, 'flags' field updated ('dataflag') 15-Apr-2005: imported Data, Dataset, Project, Site, Study, Status, Supplement metadata descriptors from the GCE Metabase ('imp_gcemetadata') 15-Apr-2005: Q/C flagging criteria applied, 'flags' field updated ('dataflag') 08-Nov-2005: imported Dataset, Project, Site, Study, Status, Supplement metadata descriptors from the GCE Metabase ('imp_gcemetadata') 08-Nov-2005: updated 66 metadata fields in the Data, Dataset, Project, Site, Status, Study, Supplement sections ('imp_gcemetadata') 06-Dec-2005: added cruise ID column Cruise at position 1 based on date information in the data set 15-Nov-2011: imported Dataset, Project, Site, Study, Status, Supplement metadata descriptors from the GCE Metabase ('imp_gcemetadata') 15-Nov-2011: updated 57 metadata fields in the Dataset, Project, Site, Status, Study, Supplement sections ('imp_gcemetadata') 17-Nov-2011: matched 30 out of 30 geographic coordinates in columns Latitude and Longitude to coordinates for registered locations in the file 'geo_locations.mat' within a distance tolerance of 1km (add_locations) 17-Nov-2011: added column 'Station' at position 6 ('addcol') 17-Nov-2011: Q/C flagging criteria applied for column(s) Station, 'flags' field updated ('dataflag') 17-Nov-2011: added geographic metadata based on matching values in columns Site and Station to site and transect names registered in 'geo_polygons.mat' and location and station names registered in 'geo_locations.mat' ('add_sitemetadata') 17-Nov-2011: updated 9 metadata fields in the Site, Status sections ('add_sitemetadata') 18-Nov-2011: imported Dataset, Project, Site, Study, Status, Supplement metadata descriptors from the GCE Metabase ('imp_gcemetadata') 18-Nov-2011: updated 57 metadata fields in the Dataset, Project, Site, Status, Study, Supplement sections ('imp_gcemetadata') 20-Jun-2013: converted dates in column(s) Date_Sampled to format yyyy-mm-dd 20-Jun-2013: flags for columns Temperature, Salinity, DPM_Sample, DPM_Control, Efficiency_Sample, Efficiency_Control, Specific_Activity and Leu_Uptake converted to data columns, flag codes updated in metadata ('flags2cols') 20-Jun-2013: updated 6 metadata fields in the Data sections ('addmeta') 20-Jun-2013: updated 15 metadata fields in the Status, Data sections to reflect attribute metadata ('updatecols') 20-Jun-2013: parsed and formatted metadata ('listmeta') B. Variable Information 1. Variable Name: column 1. Cruise column 2. Date_Sampled column 3. Time_Sampled column 4. Latitude column 5. Longitude column 6. Station column 7. Site column 8. Vessel column 9. CTD_Cast column 10. Depth_Sampled column 11. Temperature column 12. Flag_Temperature column 13. Salinity column 14. Flag_Salinity column 15. Tide column 16. Tube column 17. DPM_Sample column 18. Flag_DPM_Sample column 19. DPM_Control column 20. Flag_DPM_Control column 21. Efficiency_Sample column 22. Flag_Efficiency_Sample column 23. Efficiency_Control column 24. Flag_Efficiency_Control column 25. Specific_Activity column 26. Flag_Specific_Activity column 27. Leu_Uptake column 28. Flag_Leu_Uptake 2. Variable Definition: column 1. GCE-LTER research cruise identifier (LTER[MM][YY]) column 2. Date sample was collected column 3. Time sample was collected (GMT) column 4. Geographic latitude of sampling location column 5. Geographic longitude of sampling location column 6. Nearest nominal GCE-LTER CTD profiling station (1km geo-reference tolerance for station matching) column 7. Closest nominal GCE sampling site column 8. Vessel from which the sample was collected column 9. CTD cast number corresponding to the sample column 10. Nominal sampling depth column 11. Water temperature at the sampling location column 12. QA/QC flags for Water temperature at the sampling location (flagging criteria, where "x" is Temperature: x<0="Q", x>35="Q") column 13. Salinity of the water at the sampling location column 14. QA/QC flags for Salinity of the water at the sampling location (flagging criteria, where "x" is Salinity: x<0="I", x>36="Q") column 15. Nominal tide stage when the sample was collected column 16. Bacterial productivity sample tube number column 17. Total radioactivity in the TCA-insoluble precipitate (i.e. protein) after incubation of the live sample column 18. QA/QC flags for Total radioactivity in the TCA-insoluble precipitate (i.e. protein) after incubation of the live sample (flagging criteria, where "x" is DPM_Sample: x<0="I", x<100="Q", x>1e+007="Q", DPM_Control>(DPM_Sample.*0.025)="Q") column 19. Total radioactivity in the TCA-insoluble precipitate (i.e. protein) after incubation of the corresponding killed control column 20. QA/QC flags for Total radioactivity in the TCA-insoluble precipitate (i.e. protein) after incubation of the corresponding killed control (flagging criteria, where "x" is DPM_Control: x<0="I", x<100="Q", x>1e+007="Q", DPM_Control>(DPM_Sample.*0.025)="Q") column 21. Scintillation counting efficiency for the sample vials column 22. QA/QC flags for Scintillation counting efficiency for the sample vials (flagging criteria, where "x" is Efficiency_Sample: x<0="I", x<40="Q", x>60="Q", x>100="I") column 23. Scintillation counting efficiency for the corresponding control vial column 24. QA/QC flags for Scintillation counting efficiency for the corresponding control vial (flagging criteria, where "x" is Efficiency_Control: x<0="I", x<40="Q", x>60="Q", x>100="I") column 25. Specific activity of 3H-leucine added to the tubes column 26. QA/QC flags for Specific activity of 3H-leucine added to the tubes (flagging criteria, where "x" is Specific_Activity: x<0="I") column 27. Rate of leucine incorporation into bacterial protein calculated from rate of incorporation of 3H-leucine in incubated samples column 28. QA/QC flags for Rate of leucine incorporation into bacterial protein calculated from rate of incorporation of 3H-leucine in incubated samples (flagging criteria, where "x" is Leu_Uptake: x<0="I") 3. Units of Measurement: column 1. none column 2. yyyy-mm-dd column 3. hh:mm GMT column 4. degrees column 5. degrees column 6. none column 7. none column 8. none column 9. none column 10. none column 11. °C column 12. none column 13. PSU column 14. none column 15. none column 16. none column 17. DPM column 18. none column 19. DPM column 20. none column 21. % column 22. none column 23. % column 24. none column 25. µCi/µmol column 26. none column 27. pmol/L/hr column 28. none 4. Data Type a. Storage Type: column 1. alphanumeric column 2. alphanumeric column 3. alphanumeric column 4. floating-point column 5. floating-point column 6. alphanumeric column 7. integer column 8. alphanumeric column 9. alphanumeric column 10. alphanumeric column 11. floating-point column 12. alphanumeric column 13. floating-point column 14. alphanumeric column 15. alphanumeric column 16. integer column 17. floating-point column 18. alphanumeric column 19. floating-point column 20. alphanumeric column 21. floating-point column 22. alphanumeric column 23. floating-point column 24. alphanumeric column 25. floating-point column 26. alphanumeric column 27. floating-point column 28. alphanumeric b. Variable Codes: Site: 1 = GCE1 (Eulonia), 2 = GCE2 (Four Mile Island), 3 = GCE3 (North Sapelo), 4 = GCE4 (Meridian), 5 = GCE5 (Folly River), 6 = GCE6 (Dean Creek), 7 = GCE7 (Carrs Island), 8 = GCE8 (Alligator Creek), 9 = GCE9 (Rockdedundy Island), 10 = GCE10 (Moses Hammock) Vessel: SAV = R/V Sanannah (Skidaway Institute of Oceanography), TTSAV = T/T R/V Savannah (Skidaway Institute of Oceanography), SD = R/V Salty Dawg (University of Georgia) Depth_Sampled: S = surface sample, B = bottom sample Tide: EBB = ebbing tide, FLD = flooding tide, LW = low water, HW = high water Flag_DPM_Sample: Q = questionable value Flag_DPM_Control: Q = questionable value Flag_Efficiency_Sample: Q = questionable value Flag_Leu_Uptake: I = invalid value (out of range) c. Numeric Range: column 1. (none) column 2. (none) column 3. (none) column 4. 31.3067 to 31.5426 column 5. -81.475 to -81.2078 column 6. (none) column 7. 1 to 10 column 8. (none) column 9. (none) column 10. (none) column 11. 19.8 to 20.9 column 12. (none) column 13. 0.4 to 33.2 column 14. (none) column 15. (none) column 16. 2 to 40 column 17. 296.58 to 1539912 column 18. (none) column 19. 2896.61 to 7241.2 column 20. (none) column 21. 39.97 to 43.57 column 22. (none) column 23. 40.67 to 43.43 column 24. (none) column 25. 156 to 156 column 26. (none) column 27. -4.46 to 2604.12 column 28. (none) d. Missing Value Code: 5. Data Format a. Column Type: column 1. text column 2. text column 3. text column 4. numerical column 5. numerical column 6. text column 7. numerical column 8. text column 9. text column 10. text column 11. numerical column 12. text column 13. numerical column 14. text column 15. text column 16. numerical column 17. numerical column 18. text column 19. numerical column 20. text column 21. numerical column 22. text column 23. numerical column 24. text column 25. numerical column 26. text column 27. numerical column 28. text b. Number of Columns: 28 c. Decimal Places: column 1. 0 column 2. 0 column 3. 0 column 4. 5 column 5. 5 column 6. 0 column 7. 0 column 8. 0 column 9. 0 column 10. 0 column 11. 2 column 12. 0 column 13. 2 column 14. 0 column 15. 0 column 16. 0 column 17. 0 column 18. 0 column 19. 0 column 20. 0 column 21. 2 column 22. 0 column 23. 2 column 24. 0 column 25. 1 column 26. 0 column 27. 1 column 28. 0 6. Logical Variable Type: column 1. nominal (none) column 2. datetime (none) column 3. datetime (none) column 4. geographic coordinate (continuous) column 5. geographic coordinate (continuous) column 6. nominal (none) column 7. coded value (discrete) column 8. coded value (none) column 9. free text (none) column 10. coded value (none) column 11. data (continuous) column 12. coded value (none) column 13. data (continuous) column 14. coded value (none) column 15. coded value (none) column 16. ordinal (discrete) column 17. calculation (continuous) column 18. coded value (none) column 19. calculation (continuous) column 20. coded value (none) column 21. calculation (continuous) column 22. coded value (none) column 23. calculation (continuous) column 24. coded value (none) column 25. data (continuous) column 26. coded value (none) column 27. calculation (continuous) column 28. coded value (none) 7. Flagging Criteria: column 1. none column 2. none column 3. none column 4. x<-90="I", x<30="Q", x>32="Q", x>90="I" column 5. x<-180="I", x<-82="Q", x>-80="Q", x>180="I" column 6. flag_locationcoords(x,col_Longitude,col_Latitude,0.5,"sensitive","geo_locations.mat")="Q " column 7. x<1="I", x>10="Q" column 8. none column 9. none column 10. none column 11. x<0="Q", x>35="Q" column 12. none column 13. x<0="I", x>36="Q" column 14. none column 15. none column 16. x<1="I" column 17. x<0="I", x<100="Q", x>1e+007="Q", col_DPM_Control>(col_DPM_Sample.*0.025)="Q" column 18. none column 19. x<0="I", x<100="Q", x>1e+007="Q", col_DPM_Control>(col_DPM_Sample.*0.025)="Q" column 20. none column 21. x<0="I", x<40="Q", x>60="Q", x>100="I" column 22. none column 23. x<0="I", x<40="Q", x>60="Q", x>100="I" column 24. none column 25. x<0="I" column 26. none column 27. x<0="I" column 28. none C. Data Anomalies: Water salinity and temperature values were not measured at some stations, or could not be determined from cruise log records. DPM_Sample and DPM_Control values were flagged as Q (questionable) if the killed control value exceeded 2.5% of the live sample value. V. Supplemental Descriptors A. Data Acquisition 1. Data Forms: Paper copies of chromatograms, and standard curves used for analysis 2. Form Location: Robert E. Hodson''s laboratory, Dept. of Marine Sciences, University of Georgia, Athens, GA USA 3. Data Entry Validation: not described B. Quality Assurance/Quality Control Procedures: QA/QC flags are generated automatically according to domain criteria specified for each data column. A flag column is generated and appended to the data table if any flags are assigned to any value, listing the flags for each column on each row. C. Supplemental Materials: none D. Computer Programs: Microsoft Office XP Professional, Version 2002, copyright 1991-2001 Microsoft Corp. E. Archival Practices: Data sets and documentation are stored in structured binary (Matlab 5.x files) and delimited ASCII text formats, and archived on magnetic tape and CD at the GCE LTER Information Management Office at the University of Georgia, Athens, Georgia, USA F. Publications: not specified G. History of Data Set Usage 1. Data Request History: not specified 2. Data Set Update History: none 3. Review History: none 4. Questions and Comments from Users: none