I. Data Set Descriptors A. Title: James T. Hollibaugh. 2017. Coupling between Sediment and Water Column Populations of Ammonia Oxidizing Thaumarchaeota in the Duplin River near Sapelo Island, Georgia - Sediment Data Set. Georgia Coastal Ecosystems LTER Data Catalog (data set MIC-GCED-1702; http://gce-lter.marsci.uga.edu/public/app/dataset_details.asp?accession=MIC-GCED-1702) B. Accession Number: MIC-GCED-1702 C. Description 1. Originator(s): Name: James T. Hollibaugh Address: Dept. of Marine Sciences University of Georgia Athens, Georgia 30602-3636 Country: USA Email: aquadoc@uga.edu 2. Abstract: Populations of nitrifying organisms in the water column at Marsh Landing display a midsummer peak in the abundance of ammonia oxidizing Archaea (AOA) at the site, coinciding with a peak in nitrite concentration. Marsh Landing is at the mouth of the Duplin River, a dead-end tidal channel that drains an extensive area of salt marsh. While the lower Duplin River at Marsh Landing exchanges tidally with Doboy Sound and thus South Atlantic Bight (SAB) coastal waters, water in its upper reaches has a residence time of weeks. The work reported here had two goals: 1) test the hypothesis that the surrounding salt marsh is the source of nitrifiers seen in water samples taken at Marsh Landing; and 2) compare the seasonal dynamics of nitrifiers in surficial sediments with those in the water column. We sampled 6 stations along the ~20 km length of the Duplin River. We collected surface water samples (~0.20 m) at low- to mid-tide, monthly from April-December 2014. Sediment samples (top 1 cm) were collected at the same time from unvegetated creek bank at 2 locations on the Duplin River and from 4 locations spanning the creek bank-to-upland gradient of the saltmarsh accessible from the Teal Boardwalk. The abundance of ammonia oxidizing Archaea, Marine Group 1 Archaea (Thaumarchaeota), ammonia oxidizing Betaproteobacteria (AOB), Bacteria and Nitrospina, a nitrite oxidizing bacterium, were determined by quantitative PCR (qPCR) of DNA extracted from the samples. This data set contains the abundance estimates from April to December 2014 for sediment and water column samples, with corresponding water quality measurements (temperature, salinity and nitrogenous nutrient concentrations). 3. Study Type: Directed Study 4. Study Themes: Microbiology, General Nutrient Chemistry 5. LTER Core Areas: Other Site Research 6. Georeferences: geographic coordinates as data columns 7. Submission Date: Feb 10, 2017 D. Keywords: ammonia, ammonia oxidizing archaea, Archaea, estuaries, GCE, Georgia, Georgia Coastal Ecosystems, LTER, microbes, nitrate, nitrite, nitrogen compounds, salinity, salt marshes, Sapelo Island, sediments, temperature, Thaumarchaeota, urea, USA, water II. Research Origin Descriptors A. Overall Project Description 1. Project Title: Georgia Coastal Ecosystems LTER Project III 2. Principal Investigators: Name: Merryl Alber Address: Dept. of Marine Sciences University of Georgia Athens, Georgia 30602-3636 Country: USA Email: malber@uga.edu 3. Funding Period: Nov 01, 2012 to Nov 01, 2018 4. Objectives: The research proposed for GCE-III is designed to address how variations in salinity and inundation, driven by climate change and anthropogenic factors, affect biotic and ecosystem responses at different spatial and temporal scales, and to predict the consequences of these changes for habitat provisioning and carbon (C) sequestration across the coastal landscape. 5. Abstract: The Georgia Coastal Ecosystems (GCE) LTER is located along three adjacent sounds on the Atlantic coast and includes both intertidal marshes and estuaries. Long-term drivers of climate change, sea level rise and human alterations of the landscape will cause transitions in dominant habitat types (state changes) within the GCE domain by changing the amounts and patterns of water delivery across the landscape. These changes in water delivery can be conceptualized as presses and pulses in river inflow, local runoff, groundwater input, and tidal inundation, which will in turn manifest themselves as changes in salinity and inundation patterns in the domain. The research proposed for GCE-III is designed to address how variations in salinity and inundation, driven by climate change and anthropogenic factors, affect biotic and ecosystem responses at different spatial and temporal scales, and to predict the consequences of these changes for habitat provisioning and carbon (C) sequestration across the coastal landscape. The goals are to: 1) Track long-term changes in climate and human actions in the watershed and adjacent uplands, and evaluate the effects of these drivers on domain boundary conditions. 2) Describe temporal and spatial variability in physical, chemical, geological and biological, and to evaluate how they are affected by variations in river inflow and other boundary conditions. 3) Characterize the responses of three dominant habitats in the domain to pulses and presses in salinity and inundation. 4) Describe patterns of habitat provisioning and C sequestration and export in the GCE domain, and to evaluate how these might be affected by changes in salinity and inundation. These efforts will be synthesized into a synoptic understanding of both biotic and ecosystem responses to variations in salinity and inundation driven by climate change and human activities, which will be used to assess thresholds between habitats and the potential for state changes in the domain. 6. Funding Source: NSF OCE 1237140 B. Sub-project Description 1. Site Description a. Geographic Location: GCE10 -- Hunt Camp, Sapelo Island, Georgia, USA ML -- Marsh Landing, Sapelo Island, Georgia, USA FD_Hydro -- SINERR Flume Dock hydrographic datalogger deployment HD_Hydro -- SINERR Hunt Dock hydrographic datalogger deployment LD_Hydro -- SINERR Lower Duplin hydrographic datalogger deployment Coordinates: GCE10 -- NW: 081 17 43.82 W, 31 29 49.29 N NE: 081 15 32.07 W, 31 29 49.29 N SE: 081 15 32.07 W, 31 27 44.35 N SW: 081 17 43.82 W, 31 27 44.35 N ML -- NW: 081 17 50.80 W, 31 25 08.03 N NE: 081 17 40.58 W, 31 25 08.03 N SE: 081 17 40.58 W, 31 24 59.03 N SW: 081 17 50.80 W, 31 24 59.03 N FD_Hydro -- 81 16 03.0 W, 31 28 58.0 N HD_Hydro -- 81 16 22.8 W, 31 28 43.3 N LD_Hydro -- 81 17 45.8 W, 31 25 04.6 N b. Physiographic Region: GCE10 -- Barrier island ML -- Barrier island FD_Hydro -- Myxohaline tidal creek and marsh HD_Hydro -- Myxohaline tidal creek and marsh LD_Hydro -- Myxohaline tidal creek and marsh c. Landform Components: GCE10 -- Intertidal salt marsh bordering maritime forest ML -- Intertidal salt marsh FD_Hydro -- Intertidal marsh bordering tidal channels and creeks HD_Hydro -- Intertidal marsh bordering tidal channels and creeks LD_Hydro -- Intertidal marsh bordering tidal channels and creeks d. Hydrographic Characteristics: GCE10 -- Site borders the Mud River and contains tidal creeks and the upper reach of the Duplin River, and is subject to 2-3m semi-diurnal tides ML -- Site borders the Duplin River and is subject to 2-3m semi-diurnal tides FD_Hydro -- Site includes the Duplin River and various smaller tidal creeks and channels HD_Hydro -- Site includes the Duplin River and various smaller tidal creeks and channels LD_Hydro -- Site includes the Duplin River and various smaller tidal creeks and channels e. Topographic Attributes: GCE10 -- Flat, with elevations ranging from 0-3m above mean low tide ML -- Flat, with elevations ranging from 0-3m above mean low tide FD_Hydro -- Flat, with elevations ranging from 0-3.4m above mean low tide HD_Hydro -- Flat, with elevations ranging from 0-3.4m above mean low tide LD_Hydro -- Flat, with elevations ranging from 0-3.4m above mean low tide f. Geology, Lithology and Soils: GCE10 -- unspecified ML -- unspecified FD_Hydro -- unspecified HD_Hydro -- unspecified LD_Hydro -- unspecified g. Vegetation Communities: GCE10 -- Vegetation mostly tall and medium Spartina alterniflora, with some Juncus present. Upland heavily forested. ML -- Spartina alterniflora in low marsh, salt pan and salt-tolerant vegetation mixture in high marsh FD_Hydro -- unspecified HD_Hydro -- unspecified LD_Hydro -- unspecified h. History of Land Use and Disturbance: none recorded i. Climate: Climate summary for Sapelo Island, Georgia, based on NWS data from 1980-2010: Daily-aggregated Values: Mean (sample standard deviation) mean air temperature: 20.09°C (7.28°C) minimum air temperature: 15.02°C (7.96°C) maximum air temperature: 24.82°C (6.98°C) total precipitation: 3.26mm (10.3mm) Yearly-aggregated Daily Values: Mean (sample standard deviation) total precipitation (1980-2010): 1124mm (266mm) 2. Experimental or Sampling Design a. Design Characteristics: Near-surface water samples were collected approximately monthly at or around the mid-day at mid-tide from the Duplin River, a salt marsh-dominated, natural tidal channel adjacent to Sapelo Island, a barrier island on the Georgia (USA) coast. Water samples were collected from a boat by immersing clean, sample-rinsed, wide-mouth, 2 L, brown, aged, HDPE bottle ~20 cm below the surface (arm’s length) with the mouth facing upstream, taking care to avoid the surface microlayer. Sediment samples were collected from the boat at 2 unvegetated, creek bank locations on the Duplin River that are exposed at low tide tide. b. Permanent Plots: none c. Data Collection Duration and Frequency: not specified Beginning of Observations: Apr 09, 2014 End of Observations: Dec 01, 2014 3. Research Methods a. Field and Laboratory Methods: Method 1: Water Sampling -- Near-surface water samples were collected approximately monthly at or around the mid-day at mid-tide. Water samples were collected from a boat by immersing clean, sample-rinsed, wide-mouth, 2 L, brown, aged, HDPE bottle (Nalge Nunc; Rochester, NY) ~20 cm below the surface (arm's length) with the mouth facing upstream, taking care to avoid the surface microlayer. The sample was filtered through 0.2 m pore size, 47 mm diameter Durapore (polyvinylidene difluoride, PVDF; Millipore, Billerica MA) filters. The filter was placed in a 4 oz. WhirlPak bag (Nasco, Fort Atkinson, WI) with 1 mL of lysis buffer (0.75 M sucrose, 40 mM EDTA, 50 mM Tris; pH 8.3) then frozen at -20 °C before storage at -80 °C until analysis. One hundred milliliters of the filtrate was frozen in sterile polypropylene centrifuge tubes (model 89039-656; VWR, Radnor PA) and stored frozen for subsequent nutrient analysis. Method 2: Sediment Samples -- Sediment samples were collected from the boat at 2 unvegetated, creek bank locations on the Duplin River that are exposed at low tide tide. Another set of samples was collected at low tide from 4 sites along an upland to tidal creek gradient from a boardwalk at a nearby location (the Teal Boardwalk). Sediment samples were collected from the top 1 cm of the sediment surface in an area free of burrows or Spartina stems using plastic 5 cc syringes with the tips cut off. The lower,black sulfidic portion of the core was extruded and discarded, then the upper oxidized (gray) portion of the core was extruded into a Whirl-pak bag that was frozen at -80 oC upon return to the lab. Generally we collected 3 replicate cores from each sampling site, though only 1 core was collected at each site in April and May and the Duplin sites were not sampled in June because the tide was too high. Method 3: DNA Extraction -- Sediment samples were homogenized and 0.5 g of wet sample was placed in a MoBio® Soil Extraction kit and processed according to the manufacturer's instructions. An additional 1 mL of lysis buffer was added to filters (2 mL total) prior to DNA extraction. Briefly, a phenol:chloroform extraction method was used (Bano and Hollibaugh 2000) with a two-step enzymatic lysis (1st: lysozyme, 50 mg/mL; 2nd: 20% SDS + proteinase K, 20 mg/mL) performed in the WhirlPak bag following (Tolar et al 2013). DNA was eluted in 100 L of Tris-EDTA buffer (pH 8.0) and the eluent was used as template for quantitative PCR (qPCR). The eluent from filters and DNA retrieved from sediment samples was diluted as necessary to reduce PCR inhibition by compounds found in coastal samples. The optimal dilution for each sample was determined by qPCR amplification of Bacteria 16S rRNA (rrs) across a dilution series (1:10 to 1:1,000), then selecting the dilution yielding the highest, and thus presumably least inhibited, amplification (compared as copies L-1 of undiluted template). This dilution was used for that sample for the remaining primers and probes. Method 4: qPCR Analysis -- Primers and probes used to determine the abundance of Bacteria (rrs), Archaea (Marine Group I Archaea, or Thaumarchaeota, rrs), AOA (Archaea amoA), AOB (Betaproteobacteria amoA), and Nitrospina rrs are given in Table 1. qPCR was performed using an iCycler iQ5 (BioRad, Inc., Hercules, CA) using protocols that have been described in detail previously (Hollibaugh et al 2014, Kalanetra et al 2009, Tolar et al 2013). Gene abundance (as copies L-1 or copies g-1) was calculated from: the volume filtered or sediment wet weight, lysate volume (filters), volume of lysate purified (filters), volume of template solution yielded (both), template dilution, volume of template used per qPCR reaction, and average number of copies of the target gene for that sample as determined from qPCR results. This calculation assumes 100% extraction efficiency for all samples. qPCR reactions were run in triplicate. Replicates that differed by more than a factor of 2 from the mean of the other two replicates for the same sample were considered outliers and were not included in the average used to calculate gene abundance. Some samples did not yield a signal above the limit of detection for the primer set and are identified as Below the Limit of Detection (BLD). Method 5: Environmental Data -- Dissolved inorganic nitrogen (DIN) concentrations were determined spectrophotometrically using previously described methods for ammonia (NH4+; (Holmes et al 1998)), nitrite (NO2-) and nitrite + nitrate (NOx; (Jones 1984, Strickland and Parsons 1972). Urea was measured during the study using the diacetylmonoxime method (Rahmatullah and Boyde 1980) as modified for use in seawater by (Mulvenna and Savidge 1992). Water temperature and salinity at the sampling depth were measured by GCE-LTER personnel using a YSI data sonde. b. Protocols: Method 1: none Method 2: none Method 3: none Method 4: none Method 5: none c. Instrumentation: Method 1: none Method 2: none Method 3: none Method 4: iCycler iQ5 (BioRad, Inc., Hercules, CA) Method 5: YSI Handheld Conductivity Probe Manufacturer: YSI Instruments (Model: 30) Parameter: Conductivity (Accuracy: 1.0 uS/cm, Readability: 0.0001 to 0.1 mS/cm, Range: 0 to 200 mS/cm) Parameter: Salnity (Accuracy: 0.1 PSU, Readability: 0.01 PSU, Range: 0 to 70 PSU) Parameter: Temperature (Accuracy: 0.2°C, Readability: 0.1°C, Range: -5 to 55°C) d. Taxonomy and Systematics: Method 1: not applicable Method 2: not applicable Method 3: not applicable Method 4: not applicable Method 5: not applicable e. Speclies List: f. Permit History: Method 1: not applicable Method 2: not applicable Method 3: not applicable Method 4: not applicable Method 5: not applicable 4. Project Personnel a. Personnel: 1: James T. Hollibaugh 2: Qian Liu 3: Meredith Ross 4: Jelani Cheek 5: Corinne Sweeney 6: Bradley Tolar 7: Patrick Hagan 8: Hannah Whitby 9: Annie Bratcher 10: Erica Malagon 11: Nicole Lynn-Bell 12: Jacob Shalack 13: Caroline M. Reddy 14: John T. Walker b. Affiliations: 1: University of Georgia, Athens, Georgia 2: University of Georgia, Athens, Georgia 3: Vanderbilt Univsity 4: University of Georgia 5: University of Georgia, Athens, Georgia 6: Stanford University, Stanford, California 7: Sapelo Island National Estuarine Research Reserve, Sapelo Island, Georgia 8: Technopole Brest Iroise, Plouzane 9: University of Georgia, Athens, Georgia 10: University of Georgia, Athens, Georgia 11: University of Georgia, Athens, Georgia 12: University of Georgia Marine Institute, Sapelo Island, Georgia 13: University of Georgia, Sapelo Island, Georgia 14: U.S. Environmental Protection Agency, Raleigh-Durham, North Carolina III. Data Set Status and Accessibility A. Status 1. Latest Update: 24-Apr-2017 2. Latest Archive Date: 24-Apr-2017 3. Latest Metadata Update: 24-Apr-2017 4. Data Verification Status: New Submission B. Accessibility 1. Storage Location and Medium: Stored at GCE-LTER Data Management Office Dept. of Marine Sciences Univ. of Georgia Athens, GA 30602-3636 USA on media: electronic data download (WWW) or compact disk 2. Contact Person: Name: Wade M. Sheldon, Jr. Address: Dept. of Marine Sciences University of Georgia Athens, Georgia 30602-3636 Country: USA Email: sheldon@uga.edu 3. Copyright Restrictions: not copyrighted 4. Restrictions: All publications based on this data set must cite the contributor and Georgia Coastal Ecosystems LTER project, and two copies of the manuscript must be submitted to the GCE-LTER Information Management Office. a. Release Date: Affiliates: Feb 10, 2017, Public: Feb 10, 2018 b. Citation: Data provided by the Georgia Coastal Ecosystems Long Term Ecological Research Project, supported by funds from NSF OCE 1237140 (data set MIC-GCED-1702) c. Disclaimer: The user assumes all responsibility for errors in judgement based on interpretation of data and analyses presented in this data set. 5. Costs: free electronic data download via WWW, distribution on CD may be subject to nominal processing and handling fee IV. Data Structural Descriptors A. Data Set File 1. File Name: MIC-GCED-1702_Sediment_1_0.CSV 2. Size: 124 records 3. File Format: ASCII text (comma-separated value format) 3a. Delimiters: single comma 4. Header Information: 5 lines of ASCII text 5. Alphanumeric Attributes: 6. Quality Control Flag Codes: Q = questionable value, I = invalid value, E = estimated value, B = below detection limit 7. Authentication Procedures: 8. Calculations: 9. Processing History: Software version: GCE Data Toolbox Version 3.9.7 (04-Apr-2017) Data structure version: GCE Data Structure 1.1 (29-Mar-2001) Original data file processed: MIC-GCED-1702_Sediment.txt (124 records) Data processing history: 24-Apr-2017: new GCE Data Structure 1.1 created ('newstruct') 24-Apr-2017: 124 rows imported from ASCII data file 'MIC-GCED-1702_Sediment.txt' ('imp_ascii') 24-Apr-2017: 83 metadata fields in file header parsed ('parse_header') 24-Apr-2017: data structure validated ('gce_valid') 24-Apr-2017: Q/C flagging criteria applied, 'flags' field updated ('dataflag') 24-Apr-2017: automatically assigned study date metadata descriptors based on the range of date values in date/time columns (add_studydates) 24-Apr-2017: converted text codes in flag column(s) Flag_Archaea_amoA, Flag_Crenarchaea_16S, Flag_Bacteria_amoA, Flag_Bacteria_16S and Flag_Nitrospina_16S to QA/QC flags for data column(s) Archaea_amoA, Crenarchaea_16S, Bacteria_amoA, Bacteria_16S and Nitrospina_16S and deleted flag source columns ('cols2flags') 24-Apr-2017: manually edited data set metadata ('ui_editmetadata') 24-Apr-2017: updated 1 metadata fields in the Dataset sections ('addmeta') 24-Apr-2017: imported Dataset, Project, Site, Study, Status, Supplement metadata descriptors from the GCE Metabase ('imp_gcemetadata') 24-Apr-2017: updated 57 metadata fields in the Dataset, Project, Site, Status, Study, Supplement sections ('addmeta') 24-Apr-2017: updated 1 metadata fields in the Dataset sections ('addmeta') 24-Apr-2017: imported Dataset, Project, Site, Study, Status, Supplement metadata descriptors from the GCE Metabase ('imp_gcemetadata') 24-Apr-2017: updated 57 metadata fields in the Dataset, Project, Site, Status, Study, Supplement sections ('addmeta') 24-Apr-2017: manually edited data set metadata ('ui_editmetadata') 24-Apr-2017: edited data set title in structure and metadata ('ui_editor') 24-Apr-2017: flags for columns Archaea_amoA, Crenarchaea_16S, Bacteria_amoA, Bacteria_16S and Nitrospina_16S converted to data columns, flag codes updated in metadata ('flags2cols') 24-Apr-2017: updated 6 metadata fields in the Data sections ('addmeta') 24-Apr-2017: updated 15 metadata fields in the Status, Data sections to reflect attribute metadata ('updatecols') 24-Apr-2017: parsed and formatted metadata ('listmeta') B. Variable Information 1. Variable Name: column 1. Date column 2. StationID column 3. Latitude column 4. Longitude column 5. Archaea_amoA column 6. Flag_Archaea_amoA column 7. Crenarchaea_16S column 8. Flag_Crenarchaea_16S column 9. Bacteria_amoA column 10. Flag_Bacteria_amoA column 11. Bacteria_16S column 12. Flag_Bacteria_16S column 13. Nitrospina_16S column 14. Flag_Nitrospina_16S 2. Variable Definition: column 1. Date and time that the sample was taken column 2. Station identifier and sample replicate identifier column 3. Latitude of station sampled, decimal degree format column 4. Longitude of station sampled, decimal degree format column 5. Abundance of Archaea ammonia monooxygenase genes, thousands of copies per gram wet sediment column 6. QA/QC flags for Abundance of Archaea ammonia monooxygenase genes, thousands of copies per gram wet sediment (flagging criteria, where "x" is Archaea_amoA: x<0="I", manual) column 7. Abundance of Crenarchaea 16S rRNA genes, thousands of copies per gram wet sediment column 8. QA/QC flags for Abundance of Crenarchaea 16S rRNA genes, thousands of copies per gram wet sediment (flagging criteria, where "x" is Crenarchaea_16S: x<0="I", manual) column 9. Abundance of Betaproteobacteria ammonia monooxygenase genes, thousands of copies per gram wet sediment column 10. QA/QC flags for Abundance of Betaproteobacteria ammonia monooxygenase genes, thousands of copies per gram wet sediment (flagging criteria, where "x" is Bacteria_amoA: x<0="I", manual) column 11. Abundance of Bacteria genes, billions of copies per gram wet sediment column 12. QA/QC flags for Abundance of Bacteria genes, billions of copies per gram wet sediment (flagging criteria, where "x" is Bacteria_16S: x<0="I", manual) column 13. Abundance of Nitrospina 16S rRNA genes, thousands of copies per gram wet sediment column 14. QA/QC flags for Abundance of Nitrospina 16S rRNA genes, thousands of copies per gram wet sediment (flagging criteria, where "x" is Nitrospina_16S: x<0="I", manual) 3. Units of Measurement: column 1. YYYY-MM-DD hh:mm:ss column 2. none column 3. degrees column 4. degrees column 5. 10^3 copies/g column 6. none column 7. 10^3 copies/g column 8. none column 9. 10^3 copies/g column 10. none column 11. 10^9 copies/g column 12. none column 13. 10^3 copies/g column 14. none 4. Data Type a. Storage Type: column 1. string column 2. string column 3. floating-point column 4. floating-point column 5. floating-point column 6. string column 7. floating-point column 8. string column 9. floating-point column 10. string column 11. floating-point column 12. string column 13. floating-point column 14. string b. Variable Codes: Flag_Archaea_amoA: B = below detection limit Flag_Crenarchaea_16S: B = below detection limit Flag_Bacteria_amoA: B = below detection limit Flag_Bacteria_16S: B = below detection limit Flag_Nitrospina_16S: B = below detection limit Flag_Crenarchaea_16S: B = below detection limit Flag_Bacteria_amoA: B = below detection limit Flag_Bacteria_16S: B = below detection limit c. Numeric Range: column 1. (none) column 2. (none) column 3. 31.3952 to 31.4781 column 4. -81.2906 to -81.2737 column 5. 0.58 to 821.33 column 6. (none) column 7. 32.6 to 25821.17 column 8. (none) column 9. 1.14 to 3433.07 column 10. (none) column 11. 1.21 to 77.21 column 12. (none) column 13. 32.29 to 3621.79 column 14. (none) d. Missing Value Code: 5. Data Format a. Column Type: column 1. text column 2. text column 3. numerical column 4. numerical column 5. numerical column 6. text column 7. numerical column 8. text column 9. numerical column 10. text column 11. numerical column 12. text column 13. numerical column 14. text b. Number of Columns: 14 c. Decimal Places: column 1. 0 column 2. 0 column 3. 5 column 4. 5 column 5. 2 column 6. 0 column 7. 2 column 8. 0 column 9. 2 column 10. 0 column 11. 2 column 12. 0 column 13. 2 column 14. 0 6. Logical Variable Type: column 1. datetime (none) column 2. nominal (none) column 3. geographic coordinate (continuous) column 4. geographic coordinate (continuous) column 5. data (continuous) column 6. coded value (none) column 7. data (continuous) column 8. coded value (none) column 9. data (continuous) column 10. coded value (none) column 11. data (continuous) column 12. coded value (none) column 13. data (continuous) column 14. coded value (none) 7. Flagging Criteria: column 1. none column 2. none column 3. x<-90="I";x>90="I";x<30="Q";x>32="Q" column 4. x<-180="I";x>180="I";x<-82="Q";x>-80="Q" column 5. x<0="I";manually-assigned flags column 6. none column 7. x<0="I";manually-assigned flags column 8. none column 9. x<0="I";manually-assigned flags column 10. none column 11. x<0="I";manually-assigned flags column 12. none column 13. x<0="I";manually-assigned flags column 14. none C. Data Anomalies: V. Supplemental Descriptors A. Data Acquisition 1. Data Forms: 2. Form Location: 3. Data Entry Validation: B. Quality Assurance/Quality Control Procedures: C. Supplemental Materials: D. Computer Programs: E. Archival Practices: F. Publications: not specified G. History of Data Set Usage 1. Data Request History: not specified 2. Data Set Update History: none 3. Review History: none 4. Questions and Comments from Users: none