I. Data Set Descriptors A. Title: Brittany DeLoach McCall. 2011. Comparative transect surveys of invertebrate abundance, plant cover, and sediment characteristics in Atlantic and Gulf of Mexico salt marshes from April 2009 to September 2010. Georgia Coastal Ecosystems LTER Data Catalog (data set MLT-GCET-1106a; http://gce-lter.marsci.uga.edu/public/app/dataset_details.asp?accession=MLT-GCET-1106a) B. Accession Number: MLT-GCET-1106a C. Description 1. Originator(s): Name: Brittany DeLoach McCall Address: Department of Biology and Biochemistry University of Houston Houston, Texas 77204 Country: USA Email: bldeloach@uh.edu 2. Abstract: Understanding the relative strengths of top-down and bottom-up forces is an important key to predicting the structure of biological communities. The strength of these effects can be regulated in part by predator abundance and nutrient availability. In 2009, we hypothesized that the importance of these factors varies geographically between the southeastern Atlantic Coast and the Gulf Coast due to differences in tidal regime, and began to study this variation using a biogeographic, manipulative field experiment. Although our original purpose was to understand the structure of salt marsh arthropod food webs, BP's Deepwater Horizon spill in the Gulf Coast presented an opportunity to understand how stress from an oil spill might affect the variables that we were measuring. The fact that we had plots and transect sampling in place at multiple sites along the Gulf and East Coasts put us in a position to evaluate any impacts that might occur if oil hit some of the sites. The study was conducted at 11 sites across the Gulf Coast, from Texas to Florida, and 11 sites along the Atlantic Coast, from Florida to Maine. At each site, experimental plots were sampled and a 100m transect was sampled near the plots within 5m of the high marsh boundary. Sampling was conducted in May 2009, August 2009, and August 2010. In 2010, four extra sites were added to the existing experimental sites because of known oil contamination, and another site was added as an extra control. Only the transect sampling was conducted at these sites. This dataset contains all the transect data; data from experimental plots will be reported in a companion dataset. 3. Study Type: Graduate Thesis Study 4. Study Themes: Multi-Disciplinary Study, Aquatic Invertebrate Ecology, Organic Matter/Decomposition, Plant Ecology, Population Ecology, Terrestrial Insect Ecology 5. LTER Core Areas: Other Site Research 6. Georeferences: geographic coordinates as data columns 7. Submission Date: Jun 22, 2011 D. Keywords: abundance, abundance, Acridid, arthropods, Cecidomyiidae, Chaetopsis, Chloropidae, Delphacodes, disturbance, Dolichopodidae, ecosystems, fertilization, Foraminifera, Geukensia, insects, invertebrates, irradiance, Ischnodemus, latitude, Littoraria, longitude, marine, Megamealus, Melampus, nematodes, Oligochaetes, organic content, plants, pollution, Polychaetes, Prokelisia, salinity, sediments, Tettigoniid, Trigonotylus, Tytthus II. Research Origin Descriptors A. Overall Project Description 1. Project Title: Georgia Coastal Ecosystems LTER Project 2. Principal Investigators: Name: Merryl Alber Address: Dept. of Marine Sciences University of Georgia Athens, Georgia 30602-3636 Country: USA Email: malber@uga.edu 3. Funding Period: May 01, 2006 to Jan 01, 2013 4. Objectives: To understand the mechanisms by which variation in the quality, source and amount of both fresh and salt water create temporal and spatial variability in estuarine habitats and processes, in order to predict directional changes that will occur in response to long-term shifts in estuarine salinity patterns 5. Abstract: The Georgia Coastal Ecosystems (GCE) LTER program, located on the central Georgia coast, was established in 2000. The study domain encompasses three adjacent sounds (Altamaha, Doboy, Sapelo) and includes upland (mainland, barrier islands, marsh hammocks), intertidal (fresh, brackish and salt marsh) and submerged (river, estuary, continental shelf) habitats. Patterns and processes in this complex landscape vary spatially within and between sites, and temporally on multiple scales (tidal, diurnal, seasonal, and interannual). Overlain on this spatial and temporal variation are long-term trends caused by climate change, sea level rise, and human alterations of the landscape. These long-term trends are likely to manifest in many ways, including changes in water quality, river discharge, runoff and tidal inundation patterns throughout the estuarine landscape. The overarching goal of the GCE program is to understand the mechanisms by which variation in the quality, source and amount of both fresh and salt water create temporal and spatial variability in estuarine habitats and processes, in order to predict directional changes that will occur in response to long-term shifts in estuarine salinity patterns. The objectives of the current funding cycle are 1) to continue to document long-term patterns of environmental forcing to the coastal zone, 2) to link environmental forcing to observed spatial and temporal patterns of biogeochemical processes, primary production, community dynamics, decomposition and disturbance, 3) to investigate the underlying mechanisms by which environmental gradients along the longitudinal (freshwater-saltwater) and 4) lateral (upland-subtidal) axes of estuaries drive ecosystem change, and 5) to explore the relative importance of larval transport and the conditions of the adult environment in determining community and genetic structure across both the longitudinal and vertical gradients of the estuary. To meet these objectives, we utilize a suite of approaches including long-term monitoring of abiotic drivers and ecosystem responses; manipulative and natural experiments designed to enable us to examine the importance of key ecosystem drivers; and modeling. 6. Funding Source: NSF OCE 0620959 B. Sub-project Description 1. Site Description a. Geographic Location: SE_Coast -- Southeastern USA Coast, USA ACE_Basin_SC -- ACE_Basin_SC Anahuac_NWR -- Anahuac_NWR Aransas_NWR -- Aransas_NWR Baitshop_FL -- Baitshop_FL Bay_St_Louis_Oil_1 -- Bay_St_Louis_Oil_1 Bay_St_Louis_Oil_2 -- Bay_St_Louis_Oil_2 BI_Oil_1 -- BI_Oil_1 BI_Oil_2 -- BI_Oil_2 BI_Oil_Control -- BI_Oil_Control Boatramp_FL -- Boatramp_FL Bon_Secour_NWR -- Bon_Secour_NWR Brazoria_NWR -- Brazoria_NWR Cocodrie_LA -- Cocodrie_LA Eleven_Mi_Rd_FL -- Eleven_Mi_Rd_FL Ft_Macon_St_Park -- Ft_Macon_St_Park Grand_Isle_Oil -- Grand_Isle_Oil Grand_Isle_St_Park -- Grand_Isle_St_Park Jacques_Cousteau_NERR -- Jacques_Cousteau_NERR Plum_Island_LTER -- Plum_Island_LTER Sabine_NWR -- Sabine_NWR Sapelo_Visitor_Center -- Sapelo_Visitor_Center St_Joe_Bay_Buffer_Preserve -- St_Joe_Bay_Buffer_Preserve USC_Baruch -- USC_Baruch VCR -- VCR Waquoit_Bay_NERR -- Waquoit_Bay_NERR Wells_Bay_ME -- Wells_Bay_ME Zekes_Island_NC -- Zekes_Island_NC Coordinates: SE_Coast -- NW: 098 32 39.96 W, 43 48 01.22 N NE: 069 06 45.02 W, 43 48 01.22 N SE: 069 06 45.02 W, 24 10 02.96 N SW: 098 32 39.96 W, 24 10 02.96 N ACE_Basin_SC -- 080 26 22.3 W, 32 33 28.3 N Anahuac_NWR -- 094 33 08.2 W, 29 34 30.6 N Aransas_NWR -- 096 59 09.7 W, 28 13 12.8 N Baitshop_FL -- 081 28 32.9 W, 30 23 56.5 N Bay_St_Louis_Oil_1 -- 089 24 46.1 W, 30 15 28.0 N Bay_St_Louis_Oil_2 -- 089 24 52.0 W, 30 15 26.2 N BI_Oil_1 -- 089 55 46.4 W, 29 26 43.6 N BI_Oil_2 -- 089 54 35.9 W, 29 26 11.9 N BI_Oil_Control -- 089 59 29.6 W, 29 24 34.1 N Boatramp_FL -- 084 24 54.1 W, 29 55 26.0 N Bon_Secour_NWR -- 087 56 25.1 W, 30 14 43.9 N Brazoria_NWR -- 095 14 21.9 W, 29 03 35.9 N Cocodrie_LA -- 090 39 02.8 W, 29 16 07.4 N Eleven_Mi_Rd_FL -- 085 09 10.9 W, 29 42 29.3 N Ft_Macon_St_Park -- 076 41 58.6 W, 34 41 54.1 N Grand_Isle_Oil -- 089 57 19.7 W, 29 16 09.8 N Grand_Isle_St_Park -- 089 57 09.5 W, 29 15 43.6 N Jacques_Cousteau_NERR -- 074 19 07.7 W, 39 31 49.1 N Plum_Island_LTER -- 070 50 35.0 W, 42 44 44.4 N Sabine_NWR -- 093 24 03.4 W, 29 53 20.4 N Sapelo_Visitor_Center -- 081 21 55.4 W, 31 27 12.0 N St_Joe_Bay_Buffer_Preserve -- 085 18 06.8 W, 29 47 21.4 N USC_Baruch -- 079 12 18.8 W, 33 19 40.3 N VCR -- 075 50 16.4 W, 37 27 42.1 N Waquoit_Bay_NERR -- 070 30 22.9 W, 41 33 12.1 N Wells_Bay_ME -- 070 34 26.4 W, 43 19 16.6 N Zekes_Island_NC -- 077 56 32.3 W, 33 57 33.7 N b. Physiographic Region: SE_Coast -- unspecified ACE_Basin_SC -- unspecified Anahuac_NWR -- unspecified Aransas_NWR -- unspecified Baitshop_FL -- unspecified Bay_St_Louis_Oil_1 -- unspecified Bay_St_Louis_Oil_2 -- unspecified BI_Oil_1 -- unspecified BI_Oil_2 -- unspecified BI_Oil_Control -- unspecified Boatramp_FL -- unspecified Bon_Secour_NWR -- unspecified Brazoria_NWR -- unspecified Cocodrie_LA -- unspecified Eleven_Mi_Rd_FL -- unspecified Ft_Macon_St_Park -- unspecified Grand_Isle_Oil -- unspecified Grand_Isle_St_Park -- unspecified Jacques_Cousteau_NERR -- unspecified Plum_Island_LTER -- unspecified Sabine_NWR -- unspecified Sapelo_Visitor_Center -- unspecified St_Joe_Bay_Buffer_Preserve -- unspecified USC_Baruch -- unspecified VCR -- unspecified Waquoit_Bay_NERR -- unspecified Wells_Bay_ME -- unspecified Zekes_Island_NC -- unspecified c. Landform Components: SE_Coast -- unspecified ACE_Basin_SC -- unspecified Anahuac_NWR -- unspecified Aransas_NWR -- unspecified Baitshop_FL -- unspecified Bay_St_Louis_Oil_1 -- unspecified Bay_St_Louis_Oil_2 -- unspecified BI_Oil_1 -- unspecified BI_Oil_2 -- unspecified BI_Oil_Control -- unspecified Boatramp_FL -- unspecified Bon_Secour_NWR -- unspecified Brazoria_NWR -- unspecified Cocodrie_LA -- unspecified Eleven_Mi_Rd_FL -- unspecified Ft_Macon_St_Park -- unspecified Grand_Isle_Oil -- unspecified Grand_Isle_St_Park -- unspecified Jacques_Cousteau_NERR -- unspecified Plum_Island_LTER -- unspecified Sabine_NWR -- unspecified Sapelo_Visitor_Center -- unspecified St_Joe_Bay_Buffer_Preserve -- unspecified USC_Baruch -- unspecified VCR -- unspecified Waquoit_Bay_NERR -- unspecified Wells_Bay_ME -- unspecified Zekes_Island_NC -- unspecified d. Hydrographic Characteristics: SE_Coast -- unspecified ACE_Basin_SC -- unspecified Anahuac_NWR -- unspecified Aransas_NWR -- unspecified Baitshop_FL -- unspecified Bay_St_Louis_Oil_1 -- unspecified Bay_St_Louis_Oil_2 -- unspecified BI_Oil_1 -- unspecified BI_Oil_2 -- unspecified BI_Oil_Control -- unspecified Boatramp_FL -- unspecified Bon_Secour_NWR -- unspecified Brazoria_NWR -- unspecified Cocodrie_LA -- unspecified Eleven_Mi_Rd_FL -- unspecified Ft_Macon_St_Park -- unspecified Grand_Isle_Oil -- unspecified Grand_Isle_St_Park -- unspecified Jacques_Cousteau_NERR -- unspecified Plum_Island_LTER -- unspecified Sabine_NWR -- unspecified Sapelo_Visitor_Center -- unspecified St_Joe_Bay_Buffer_Preserve -- unspecified USC_Baruch -- unspecified VCR -- unspecified Waquoit_Bay_NERR -- unspecified Wells_Bay_ME -- unspecified Zekes_Island_NC -- unspecified e. Topographic Attributes: SE_Coast -- unspecified ACE_Basin_SC -- unspecified Anahuac_NWR -- unspecified Aransas_NWR -- unspecified Baitshop_FL -- unspecified Bay_St_Louis_Oil_1 -- unspecified Bay_St_Louis_Oil_2 -- unspecified BI_Oil_1 -- unspecified BI_Oil_2 -- unspecified BI_Oil_Control -- unspecified Boatramp_FL -- unspecified Bon_Secour_NWR -- unspecified Brazoria_NWR -- unspecified Cocodrie_LA -- unspecified Eleven_Mi_Rd_FL -- unspecified Ft_Macon_St_Park -- unspecified Grand_Isle_Oil -- unspecified Grand_Isle_St_Park -- unspecified Jacques_Cousteau_NERR -- unspecified Plum_Island_LTER -- unspecified Sabine_NWR -- unspecified Sapelo_Visitor_Center -- unspecified St_Joe_Bay_Buffer_Preserve -- unspecified USC_Baruch -- unspecified VCR -- unspecified Waquoit_Bay_NERR -- unspecified Wells_Bay_ME -- unspecified Zekes_Island_NC -- unspecified f. Geology, Lithology and Soils: SE_Coast -- unspecified ACE_Basin_SC -- unspecified Anahuac_NWR -- unspecified Aransas_NWR -- unspecified Baitshop_FL -- unspecified Bay_St_Louis_Oil_1 -- unspecified Bay_St_Louis_Oil_2 -- unspecified BI_Oil_1 -- unspecified BI_Oil_2 -- unspecified BI_Oil_Control -- unspecified Boatramp_FL -- unspecified Bon_Secour_NWR -- unspecified Brazoria_NWR -- unspecified Cocodrie_LA -- unspecified Eleven_Mi_Rd_FL -- unspecified Ft_Macon_St_Park -- unspecified Grand_Isle_Oil -- unspecified Grand_Isle_St_Park -- unspecified Jacques_Cousteau_NERR -- unspecified Plum_Island_LTER -- unspecified Sabine_NWR -- unspecified Sapelo_Visitor_Center -- unspecified St_Joe_Bay_Buffer_Preserve -- unspecified USC_Baruch -- unspecified VCR -- unspecified Waquoit_Bay_NERR -- unspecified Wells_Bay_ME -- unspecified Zekes_Island_NC -- unspecified g. Vegetation Communities: SE_Coast -- unspecified ACE_Basin_SC -- unspecified Anahuac_NWR -- unspecified Aransas_NWR -- unspecified Baitshop_FL -- unspecified Bay_St_Louis_Oil_1 -- unspecified Bay_St_Louis_Oil_2 -- unspecified BI_Oil_1 -- unspecified BI_Oil_2 -- unspecified BI_Oil_Control -- unspecified Boatramp_FL -- unspecified Bon_Secour_NWR -- unspecified Brazoria_NWR -- unspecified Cocodrie_LA -- unspecified Eleven_Mi_Rd_FL -- unspecified Ft_Macon_St_Park -- unspecified Grand_Isle_Oil -- unspecified Grand_Isle_St_Park -- unspecified Jacques_Cousteau_NERR -- unspecified Plum_Island_LTER -- unspecified Sabine_NWR -- unspecified Sapelo_Visitor_Center -- unspecified St_Joe_Bay_Buffer_Preserve -- unspecified USC_Baruch -- unspecified VCR -- unspecified Waquoit_Bay_NERR -- unspecified Wells_Bay_ME -- unspecified Zekes_Island_NC -- unspecified h. History of Land Use and Disturbance: none recorded i. Climate: Climate summary for Sapelo Island, Georgia, based on NWS data from 1980-2010: Daily-aggregated Values: Mean (sample standard deviation) mean air temperature: 20.09°C (7.28°C) minimum air temperature: 15.02°C (7.96°C) maximum air temperature: 24.82°C (6.98°C) total precipitation: 3.26mm (10.3mm) Yearly-aggregated Daily Values: Mean (sample standard deviation) total precipitation (1980-2010): 1124mm (266mm) 2. Experimental or Sampling Design a. Design Characteristics: A survey of vegetation, soil parameters, and invertebrate abundances was done along a 100m transect through the Spartina alterniflora zone within 5m of the high marsh boundary. Sampling was also conducted in four experimental plots at each site. b. Permanent Plots: Four 3x2m plots were established in April 2009 in the Spartina alterniflora zone, originating at the high marsh boundary and demarcated by a fence of Tenax black plastic netting ziptied to pvc stakes. Experimental treatments of fertilizer addition, wrack addition, fertilizer & wrack addition, and no addition (control) were randomly applied to the plots. The plot treatments were maintained in August 2009 and May 2010. c. Data Collection Duration and Frequency: Transects were sampled in May 2009, August 2009, and August 2010. Plots were sampled in August of 2009 and 2010. Insect samples consisting of five pooled 10 second collections were taken from the lower 1x1m of the experimental plots using a DVAC suction sampler, a pooled insect sample of eight 10 second collections was taken along a 100m transect using a DVAC, a pooled soil sample of 4 cores was collected along a 100m transect, a pooled benthic invertebrate sample of 4 cores was collected along a 100m transect (2010 only), a pooled sample of 8 leaves was randomly collected along a 100m transect, up to 50 individuals of Littoraria were collected from each site (2010 only), up to 10 individuals of Uca were collected from each site (2010 only), up to 10 individuals of Geukensia were collected from each site (2010 only). Beginning of Observations: Apr 25, 2009 End of Observations: Sep 15, 2010 3. Research Methods a. Field and Laboratory Methods: Method 1: Sampling Plot Installation -- Four 3x2m plots were established in April 2009 in the Spartina alterniflora zone, originating at the high marsh boundary and demarcated by a fence of Tenax black plastic netting ziptied to pvc stakes. Experimental treatments of fertilizer addition, wrack addition, fertilizer & wrack addition, and no addition (control) were randomly applied to the plots. The plot treatments were maintained in August 2009 and May 2010. Method 2: GPS Survey of Plots -- GPS points were taken at the base of each plot, along with the beginning and the end of the sampling transect. Method 3: Insect Sampling -- Samples were collected with a DVAC suction sampler with a modified head of area 0.0706 m2. Samples consisted of multiple 10 second sucks pooled together. Net was removed and placed into a 1qt mason jar with ethanol. Method 4: Soil Sampling -- Within the 100m transect, four spots at roughly 25 m intervals were randomly chosen. A metal trowel was used to remove soil at least 10cm deep. Collections were pooled into one sample (about 300 mL). Method 5: Oiled Soil Sampling -- Within the 100m transect, four spots at roughly 25 m intervals were randomly chosen. A metal trowel was used to remove soil at least 10cm deep. Collections were pooled into one sample (about 500 ml), placed into a 0.5pt mason jar, and kept on ice until able to go into the freezer. Method 6: Benthic Sampling -- Within the 100m transect, four spots at roughly 25 m intervals were randomly chosen. A metal trowel was used to remove soil at least 10cm deep. Collections were pooled into one sample (about 200 ml) and rinsed through a 300micrometer sieve with water. Material retained on the sieve was placed into jars with ethanol. Method 7: Vegetation measurements -- Vegetation measurements were taken along the transect (in both 2009 and 2010) and in the plots (2010 only). Within the 100m transect, four 0.5 x 0.5 m quadrats were haphazardly located at roughly 25 m intervals. Within the plots, quadrats were centered within the lower 1m2 in the experimental plots. Quadrats were divided into 100 cells with string. At each quadrat, the percent cover of live Spartina was evaluated by counting how many cells out of 100 the plant appeared in. The percent cover of dead plant material was also evaluated by counting how many cells out of 100 the material appeared in. The height of the tallest plant in the quadrat was measured with a meter stick by pulling the living leaves up to their tallest point. Measurements of light intensity were taken with a sunwand above the vegetation and at the marsh surface to measure light interception. Method 8: Leaf Sampling -- Leaves were sampled from each vegetation quadrat along the 100m transect. At each quadrat, the 3rd leaf from two different Spartina plants was collected. All eight leaves from the transect were pooled together and placed into a sealed envelope and dried. Method 9: Benthic invertebrates -- Within the 100m transect, six quadrats (0.25 x 0.25m, 0.5 x 0.5m or 1 x 1 m, depending on invertebrate density) were randomly dropped at roughly 20 m intervals. At each quadrat, we counted the number of snails, Geukensia mussels, and crab burrows. The diameter of the first 30 burrows (> 3 mm in diameter) present was measured. Method 10: Littoraria collection -- Along the 100m transect, the first 50 Littoraria observed were collected, rinsed with water, and preserved in mason jars with ethanol. If none were found in the transect, they were collected from neighboring areas of the marsh. Method 11: Uca collection -- Along the 100m transect, up to 10 Uca fiddler crabs were collected, rinsed with water, and preserved in mason jars with ethanol. If none were found in the transect, they were collected from neighboring areas of the marsh. Method 12: Geukensia collection -- Along the 100m transect, up to 10 Geukensia mussels were collected, rinsed with water, and preserved in mason jars with ethanol. If none were found in the transect, they were collected from neighboring areas of the marsh. Method 13: Plot Fertilization -- Plots were fertilized in May 2009, August 2009, May 2010, and August 2010 using Agriform slow-release planting tablets (20-10-5). We fertilized at a rate of eight 21g tablets per m2, which placed around 33.6g of nitrogen per m2 into the plots. The tablets were placed at least 10cm below the surface. Control and wrack addition plots were probed with a pvc pipe to mimic the disturbance caused by fertilizing. Method 14: Plot Wrack Manipulation -- Plots had dead plant material added to them until approximately twice the ambient level was maintained. In some areas, ambient levels of standing dead were too high to achieve a concentration double that, so around 5 gallons of wrack and thatch were added. Wrack and thatch were placed evenly throughout the plot around the mid to base of the stems. Vegetation in the control and fertilizer addition plots was tousled to mimic the disturbance caused by wrack addition. Method 15: Soil organic matter -- The soil sample was weighed wet and then air dried until returned to the laboratory. In the lab, we dried soils at 60°C for 4 days and ground and mixed dry soils with a mortar and pestle. Soil organic content was measured as loss on ignition of the dried soils in a muffle furnace at 440°C for 24hr. Method 16: Soil pore water -- The soil sample was weighed wet and then air dried until returned to the laboratory. In the lab, we dried soils at 60°C for 4 days, weighed the soils, and calculated soil water content gravimetrically. Method 17: Soil Pore Water Salinity -- The soil sample was weighed wet and then air dried until returned to the laboratory. In the lab, we dried soils at 60°C for 4 days and ground and mixed dry soils with a mortar and pestle. Dried soils were rehydrated with a known volume of distilled water, the salinity measured after 24h, and back-calculated to the original water content to estimate initial pore water salinity. Method 18: Soil Bitumen Content -- After the spill in 2010, a second set of soil samples were collected, placed into glass jars, and put on ice until returned to the laboratory. In the lab, a subsample was dried at 40°C for 6 days and ground and mixed with a mortar and pestle. A soxhlet extraction followed by rotary evaporation was performed on the dried soils to quantify organic bitumen. Method 19: Leaf Nitrogen Content -- The collected leaf samples were air-dried until returned to the laboratory, where they were lyophilized for 72hr, ground with a Spex 8000M Mixer/Mill, and analyzed for nitrogen content at the UGA Chemical Analysis Lab. b. Instrumentation: Method 1: none Method 2: GPS receiver Manufacturer: Garmin (Model: GPSMAP 76CSx) Parameter: altitude (Accuracy: 3m, Readability: 0.3m, ) Parameter: latitude (Accuracy: 3-5m, Readability: 0.06 degrees, Range: 0-90 degrees) Parameter: longitude (Accuracy: 3-5m, Readability: 0.06 degrees, Range: 0-180 degrees) Method 3: D-Vac vacuum insect collector Manufacturer: Rincon-Vitova (Model: 24) Method 4: none Method 5: none Method 6: none Method 7: PVC ruler Parameter: length SunScan Canopy Analysis System Manufacturer: Delta-T Devices (Model: SS1-UM-1.05) Parameter: PAR intensity (Accuracy: 10%, Readability: 0.3 mmol/m^2/s, Range: 0-2500 mmol/m^2/s) Method 8: Drying oven Manufacturer: Fisher Scientific (Model: 750G) Method 9: none Method 10: none Method 11: none Method 12: none Method 13: none Method 14: none Method 15: Drying oven Manufacturer: Fisher Scientific (Model: 750G) Muffle furnace Method 16: Drying oven Manufacturer: Fisher Scientific (Model: 750G) Electronic balance Parameter: mass Method 17: Drying oven Manufacturer: Fisher Scientific (Model: 750G) Electronic balance Parameter: mass Refractometer Manufacturer: Vista (Model: A355ATC) Parameter: Salinity (Accuracy: 1 PSU, Readability: 1 PSU, Range: 0-100 PSU) Method 18: Drying oven Manufacturer: Fisher Scientific (Model: 750G) Electronic balance Parameter: mass Method 19: Laboratory Mill Manufacturer: SPEX (Model: SamplePrep 8000M Mixer/Mill) c. Taxonomy and Systematics: Method 1: not applicable Method 2: not applicable Method 3: not applicable Method 4: not applicable Method 5: not applicable Method 6: not applicable Method 7: not applicable Method 8: not applicable Method 9: not applicable Method 10: not applicable Method 11: not applicable Method 12: not applicable Method 13: not applicable Method 14: not applicable Method 15: not applicable Method 16: not applicable Method 17: not applicable Method 18: not applicable Method 19: not applicable d. Permit History: Method 1: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 2: not applicable Method 3: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 4: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 5: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 6: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 7: not applicable Method 8: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 9: not applicable Method 10: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 11: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 12: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 13: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 14: USFWS SUP for Aransas National Wildlife Refuge, Brazoria National Wildlife Refuge, Anahuac National Wildlife Refuge, Sabine National Wildlife Refuge, and Bon Secour National Wildlife Refuge; State of Louisiana Collection Permit for Grand Isle State Park; South Carolina Department of Natural Resources Research Permit for ACE Basin; Research permit from NC National Estuarine Research Reserve; NC Division of Parks and Recreation Research Permit; The Nature Conservancy Research Permit Method 15: not applicable Method 16: not applicable Method 17: not applicable Method 18: not applicable Method 19: not applicable 4. Project Personnel a. Personnel: 1: Brittany DeLoach McCall 2: Steven C. Pennings 3: Huy Vu 4: Kazimierz Wieski 5: Hongyu Guo 6: Michael Richardson 7: Brittany DeLong 8: Claudia Grimm 9: Annika Stark b. Affiliations: 1: University of Houston, Houston, Texas 2: University of Houston, Houston, Texas 3: University of Houston, Houston, Texas 4: University of Houston, Houston, Texas 5: University of Houston, Houston, Texas 6: University of Houston, Houston, Texas 7: University of Houston 8: Christian-Albrechts University 9: Christian-Albrechts University III. Data Set Status and Accessibility A. Status 1. Latest Update: 19-Feb-2012 2. Latest Archive Date: 10-Dec-2011 3. Latest Metadata Update: 19-Feb-2012 4. Data Verification Status: New Submission B. Accessibility 1. Storage Location and Medium: Stored at GCE-LTER Data Management Office Dept. of Marine Sciences Univ. of Georgia Athens, GA 30602-3636 USA on media: electronic data download (WWW) or compact disk 2. Contact Person: Name: Wade M. Sheldon, Jr. Address: Dept. of Marine Sciences University of Georgia Athens, Georgia 30602-3636 Country: USA Email: sheldon@uga.edu 3. Copyright Restrictions: not copyrighted 4. Restrictions: All publications based on this data set must cite the contributor and Georgia Coastal Ecosystems LTER project, and two copies of the manuscript must be submitted to the GCE-LTER Information Management Office. a. Release Date: GCE LTER Affiliates: Jun 22, 2011, Public: Jan 01, 2012 b. Citation: Data provided by the Georgia Coastal Ecosystems Long Term Ecological Research Project, supported by funds from NSF OCE 0620959 (data set MLT-GCET-1106a) c. Disclaimer: The user assumes all responsibility for errors in judgement based on interpretation of data and analyses presented in this data set. 5. Costs: free electronic data download via WWW, distribution on CD may be subject to nominal processing and handling fee IV. Data Structural Descriptors A. Data Set File 1. File Name: MLT-GCET-1106a_1_1.CSV 2. Size: 74 records 3. File Format: ASCII text (comma-separated value format) 3a. Delimiters: single comma 4. Header Information: 5 lines of ASCII text 5. Alphanumeric Attributes: 6. Quality Control Flag Codes: 7. Authentication Procedures: 8. Calculations: Light_Intercepted: Light_Intercepted = (PAR_Above_Canopy - PAR_Below_Canopy)/(PAR_Above_Canopy) 9. Processing History: Software version: GCE Data Toolbox Version 3.30 (09-Feb-2012) Data structure version: GCE Data Structure 1.1 (29-Mar-2001) Original data file processed: MLT-GCET-1106a.TXT (74 records) Data processing history: 10-Dec-2011: new GCE Data Structure 1.1 created ('newstruct') 10-Dec-2011: 74 rows imported from ASCII data file 'MLT-GCET-1106a.TXT' ('imp_ascii') 10-Dec-2011: 13 metadata fields in file header parsed ('parse_header') 10-Dec-2011: data structure validated ('gce_valid') 10-Dec-2011: Q/C flagging criteria applied, 'flags' field updated ('dataflag') 10-Dec-2011: automatically assigned study date metadata descriptors based on the range of date values in date/time columns (add_studydates) 10-Dec-2011: imported Dataset, Project, Site, Study, Status, Supplement metadata descriptors from the GCE Metabase ('imp_gcemetadata') 10-Dec-2011: updated 57 metadata fields in the Dataset, Project, Site, Status, Study, Supplement sections ('imp_gcemetadata') 19-Feb-2012: updated 7 metadata fields in the Data, Status sections ('exp_ascii') 19-Feb-2012: updated 15 metadata fields in the Status, Data sections to reflect attribute metadata ('updatecols') 19-Feb-2012: parsed and formatted metadata ('listmeta') B. Variable Information 1. Variable Name: column 1. Location column 2. Latitude column 3. Longitude column 4. Day column 5. Month column 6. Year column 7. Oil_Presence column 8. Soil_Organic_Content column 9. Soil_Water_Content column 10. Soil_Salinity column 11. Soil_Bitumen column 12. Leaf_Nitrogen column 13. Spartina_Cover column 14. Dead_Plant_Cover column 15. Spartina_Height column 16. PAR_Above_Canopy column 17. PAR_Below_Canopy column 18. Light_Intercepted column 19. Littoraria_Density column 20. Littoraria_Height column 21. Melampus_Density column 22. Black_Snail_Density column 23. Geukensia_Density column 24. Crab_Burrow_Density column 25. Crab_Burrow_Diameter column 26. Tettigoniid_Abundance column 27. Foraminifera_Count_Sediment column 28. Nematode_Count_Sediment column 29. Polychaete_Count_Sediment column 30. Oligochaete_Count_Sediment column 31. Unident_Worm_Count_Sediment column 32. Snail_Count_Sediment column 33. Bivalve_Count_Sediment column 34. Other_Mollusc_Count_Sediment column 35. Crab_Count_Sediment column 36. Amphipod_Count_Sediment column 37. Insect_Count_Sediment column 38. Spider_Count_Sediment column 39. Other_Arthropod_Count_Sediment column 40. Unidentified_Infauna_Count_Sediment column 41. Trigonotylus_uhleri_Count_DVac column 42. Leafhopper_Count_DVac column 43. Prokelisia_Count_DVac column 44. Delphacodes_penedetecta_Count_DVac column 45. Megamealus_Count_DVac column 46. Immature_Delphacids_Count_DVac column 47. Other_Planthopper_Count_DVac column 48. Ischnodemus_Count_DVac column 49. Tettigonid_Count_DVac column 50. Acridid_Count_DVac column 51. Thrips_Count_DVac column 52. Weevil_Count_DVac column 53. Chaetopsis_Fly_Count_DVac column 54. Chloropidae_Count_DVac column 55. Cecidomyiidae_Count_DVac column 56. Dolichopodidae_Count_DVac column 57. Other_Herbivore_Count_DVac column 58. Tythus_vagus_Count_DVac column 59. Naemia_Count_DVac column 60. Hogna_Count_DVac column 61. Pardosa_Count_DVac column 62. Marpissa_Count_DVac column 63. Other_Salticid_Count_DVac column 64. Clubionid_Count_DVac column 65. Tetragnatha_Count_DVac column 66. Grammonota_Count_DVac column 67. Other_Spider_Count_DVac column 68. Other_Predator_Count_DVac column 69. Wasp_Count_DVac column 70. Collembola_Count_DVac column 71. Brachycera_Count_DVac column 72. Nematocera_Count_DVac 2. Variable Definition: column 1. Name of the sampling location column 2. Geographic latitude of the sampling location column 3. Geographic longitude of the sampling location column 4. Calendar day site was sampled column 5. Calendar month site was sampled column 6. Calendar year site was sampled column 7. Presence of obvious oil in water and on plants and soil column 8. Soil organic content as determined by loss of dry mass after ignition in a muffle furnace column 9. Soil water content as determined by difference between wet weight and dry weight column 10. Salinity of pore water column 11. Oil content of soil by mass column 12. Nitrogen content of Spartina alterniflora leaf column 13. Percent cover of Spartina in 0.5 x 0.5 m quadrat column 14. Percent cover of dead plant material in 0.5 x 0.5 m quadrat column 15. Height of tallest Spartina alterniflora plant column 16. PAR reading above plant canopy column 17. PAR reading at soil level column 18. Fraction of light intercepted by the canopy column 19. Average number of littoraria per square meter column 20. Average spire height of Littoraria from transect column 21. Average number of Melampus per square meter column 22. Average number of black snails per square meter column 23. Average number of Geukensia per square meter column 24. Average number of crab burrows per square meter column 25. Average diameter of crab burrows from transect column 26. Estimate of tettigoniid abundance column 27. Number of Foraminifera in pooled sediment sample column 28. Number of nematodes in pooled sediment sample column 29. Number of polychaetes in pooled sediment sample column 30. Number of oligochaetes in pooled sediment sample column 31. Number of unidents in pooled sediment sample Number of Worms in pooled sediment sample column 32. Number of snails in pooled sediment sample column 33. Number of bivalves in pooled sediment sample column 34. Number of other molluscs in pooled sediment sample column 35. Number of crabs in pooled sediment sample column 36. Number of amphipods in pooled sediment sample column 37. Number of insects in pooled sediment sample column 38. Number of spiders in pooled sediment sample column 39. Number of other arthropods in pooled sediment sample column 40. Number of unidentified infauna in pooled sediment sample column 41. Number of Trigonotylus uhleri in pooled DVac sample column 42. Number of leafhoppers in pooled DVac sample column 43. Number of Prokelisia in pooled DVac sample column 44. Number of Delphacodes penedetecta in pooled DVac sample column 45. Number of Megamealus in pooled DVac sample column 46. Number of immature Delphacids in pooled DVac sample column 47. Number of other Planthoppers in pooled DVac sample column 48. Number of Ischnodemus in pooled DVac sample column 49. Number of Tettigonid in pooled DVac sample column 50. Number of Acridid in pooled DVac sample column 51. Number of Thrips in pooled DVac sample column 52. Number of weevils in pooled DVac sample column 53. Number of Chaetopsis fly in pooled DVac sample column 54. Number of Chloropidae in pooled DVac sample column 55. Number of Cecidomyiidae in pooled DVac sample column 56. Number of Dolichopodidae in pooled DVac sample column 57. Number of other herbivores in pooled DVac sample column 58. Number of Tythus vagus in pooled DVac sample column 59. Number of Naemia in pooled DVac sample column 60. Number of Hogna in pooled DVac sample column 61. Number of Pardosa in pooled DVac sample column 62. Number of Marpissa in pooled DVac sample column 63. Number of other Salticid in pooled DVac sample column 64. Number of Clubionid in pooled DVac sample column 65. Number of Tetragnatha in pooled DVac sample column 66. Number of Grammonota in pooled DVac sample column 67. Number of other spiders in pooled DVac sample column 68. Number of other predators in pooled DVac sample column 69. Number of wasps in pooled DVac sample column 70. Number of Collembola in pooled DVac sample column 71. Number of Brachycera in pooled DVac sample column 72. Number of Nematocera in pooled DVac sample 3. Units of Measurement: column 1. none column 2. degrees column 3. degrees column 4. D column 5. M column 6. YYYY column 7. none column 8. percent column 9. percent column 10. PSU column 11. percent column 12. percent column 13. percent column 14. percent column 15. cm column 16. µE/m^2/s column 17. µE/m^2/s column 18. ratio column 19. count/m^2 column 20. mm column 21. count/m^2 column 22. count/m^2 column 23. count/m^2 column 24. count/m^2 column 25. cm column 26. none column 27. count column 28. count column 29. count column 30. count column 31. count column 32. count column 33. count column 34. count column 35. count column 36. count column 37. count column 38. count column 39. count column 40. count column 41. count column 42. count column 43. count column 44. count column 45. count column 46. count column 47. count column 48. count column 49. count column 50. count column 51. count column 52. count column 53. count column 54. count column 55. count column 56. count column 57. count column 58. count column 59. count column 60. count column 61. count column 62. count column 63. count column 64. count column 65. count column 66. count column 67. count column 68. count column 69. count column 70. count column 71. count column 72. count 4. Data Type a. Storage Type: column 1. alphanumeric column 2. floating-point column 3. floating-point column 4. integer column 5. integer column 6. integer column 7. integer column 8. floating-point column 9. floating-point column 10. floating-point column 11. floating-point column 12. floating-point column 13. floating-point column 14. floating-point column 15. floating-point column 16. floating-point column 17. floating-point column 18. floating-point column 19. floating-point column 20. floating-point column 21. floating-point column 22. floating-point column 23. floating-point column 24. floating-point column 25. floating-point column 26. integer column 27. integer column 28. integer column 29. integer column 30. integer column 31. integer column 32. integer column 33. integer column 34. integer column 35. integer column 36. integer column 37. integer column 38. integer column 39. integer column 40. integer column 41. integer column 42. integer column 43. integer column 44. integer column 45. integer column 46. integer column 47. integer column 48. integer column 49. integer column 50. integer column 51. integer column 52. integer column 53. integer column 54. integer column 55. integer column 56. integer column 57. integer column 58. integer column 59. integer column 60. integer column 61. integer column 62. integer column 63. integer column 64. integer column 65. integer column 66. integer column 67. integer column 68. integer column 69. integer column 70. integer column 71. integer column 72. integer b. Variable Codes: Oil_Presence: 0 = no oil observed, 1 = oil observed Tettigoniid_Abundance: 1 = none, 2 = rare, 3 = common, 4 = abundant c. Numeric Range: column 1. (none) column 2. 28.2202 to 43.3213 column 3. -96.986 to -70.5063 column 4. 1 to 31 column 5. 4 to 9 column 6. 2009 to 2010 column 7. 0 to 1 column 8. 0.3 to 79.7 column 9. 18.6 to 88.9 column 10. 4.1 to 86.4 column 11. 0.013 to 0.962 column 12. 0.71 to 2.14 column 13. 21.3 to 100 column 14. 5.8 to 100 column 15. 24.25 to 131 column 16. 15.9 to 2060.3 column 17. 5 to 1550.1 column 18. 0.248 to 0.915 column 19. 0 to 206.33 column 20. 10.49 to 26.14 column 21. 0 to 308.667 column 22. 0 to 9.333 column 23. 0 to 13 column 24. 0 to 51.33 column 25. 0.5 to 2.6 column 26. 1 to 4 column 27. 0 to 146 column 28. 0 to 0 column 29. 0 to 6 column 30. 0 to 43 column 31. 0 to 26 column 32. 0 to 14 column 33. 0 to 2 column 34. 0 to 1 column 35. 0 to 1 column 36. 0 to 4 column 37. 0 to 14 column 38. 0 to 8 column 39. 0 to 4 column 40. 0 to 5 column 41. 0 to 69 column 42. 0 to 49 column 43. 0 to 1261 column 44. 0 to 138 column 45. 0 to 144 column 46. 0 to 3300 column 47. 0 to 16 column 48. 0 to 35 column 49. 0 to 7 column 50. 0 to 3 column 51. 0 to 6 column 52. 0 to 1 column 53. 0 to 28 column 54. 14 to 1408 column 55. 0 to 90 column 56. 0 to 21 column 57. 0 to 117 column 58. 0 to 100 column 59. 0 to 1 column 60. 0 to 9 column 61. 0 to 33 column 62. 0 to 12 column 63. 0 to 17 column 64. 0 to 21 column 65. 0 to 39 column 66. 0 to 94 column 67. 0 to 120 column 68. 0 to 28 column 69. 0 to 172 column 70. 0 to 2572 column 71. 5 to 1564 column 72. 0 to 1144 d. Missing Value Code: 5. Data Format a. Column Type: column 1. text column 2. numerical column 3. numerical column 4. numerical column 5. numerical column 6. numerical column 7. numerical column 8. numerical column 9. numerical column 10. numerical column 11. numerical column 12. numerical column 13. numerical column 14. numerical column 15. numerical column 16. numerical column 17. numerical column 18. numerical column 19. numerical column 20. numerical column 21. numerical column 22. numerical column 23. numerical column 24. numerical column 25. numerical column 26. numerical column 27. numerical column 28. numerical column 29. numerical column 30. numerical column 31. numerical column 32. numerical column 33. numerical column 34. numerical column 35. numerical column 36. numerical column 37. numerical column 38. numerical column 39. numerical column 40. numerical column 41. numerical column 42. numerical column 43. numerical column 44. numerical column 45. numerical column 46. numerical column 47. numerical column 48. numerical column 49. numerical column 50. numerical column 51. numerical column 52. numerical column 53. numerical column 54. numerical column 55. numerical column 56. numerical column 57. numerical column 58. numerical column 59. numerical column 60. numerical column 61. numerical column 62. numerical column 63. numerical column 64. numerical column 65. numerical column 66. numerical column 67. numerical column 68. numerical column 69. numerical column 70. numerical column 71. numerical column 72. numerical b. Number of Columns: 72 c. Decimal Places: column 1. 0 column 2. 5 column 3. 5 column 4. 0 column 5. 0 column 6. 0 column 7. 0 column 8. 1 column 9. 1 column 10. 1 column 11. 3 column 12. 2 column 13. 1 column 14. 1 column 15. 2 column 16. 2 column 17. 2 column 18. 3 column 19. 2 column 20. 2 column 21. 3 column 22. 3 column 23. 3 column 24. 2 column 25. 2 column 26. 0 column 27. 0 column 28. 0 column 29. 0 column 30. 0 column 31. 0 column 32. 0 column 33. 0 column 34. 0 column 35. 0 column 36. 0 column 37. 0 column 38. 0 column 39. 0 column 40. 0 column 41. 0 column 42. 0 column 43. 0 column 44. 0 column 45. 0 column 46. 0 column 47. 0 column 48. 0 column 49. 0 column 50. 0 column 51. 0 column 52. 0 column 53. 0 column 54. 0 column 55. 0 column 56. 0 column 57. 0 column 58. 0 column 59. 0 column 60. 0 column 61. 0 column 62. 0 column 63. 0 column 64. 0 column 65. 0 column 66. 0 column 67. 0 column 68. 0 column 69. 0 column 70. 0 column 71. 0 column 72. 0 6. Logical Variable Type: column 1. nominal (none) column 2. geographic coordinate (continuous) column 3. geographic coordinate (continuous) column 4. datetime (discrete) column 5. datetime (discrete) column 6. datetime (discrete) column 7. logical (discrete) column 8. calculation (continuous) column 9. calculation (continuous) column 10. calculation (continuous) column 11. calculation (continuous) column 12. calculation (continuous) column 13. calculation (continuous) column 14. calculation (continuous) column 15. data (continuous) column 16. data (continuous) column 17. data (continuous) column 18. calculation (continuous) column 19. calculation (continuous) column 20. calculation (continuous) column 21. calculation (continuous) column 22. calculation (continuous) column 23. calculation (continuous) column 24. calculation (continuous) column 25. data (continuous) column 26. coded value (discrete) column 27. data (discrete) column 28. data (discrete) column 29. data (discrete) column 30. data (discrete) column 31. data (discrete) column 32. data (discrete) column 33. data (discrete) column 34. data (discrete) column 35. data (discrete) column 36. data (discrete) column 37. data (discrete) column 38. data (discrete) column 39. data (discrete) column 40. data (discrete) column 41. data (discrete) column 42. data (discrete) column 43. data (discrete) column 44. data (discrete) column 45. data (discrete) column 46. data (discrete) column 47. data (discrete) column 48. data (discrete) column 49. data (discrete) column 50. data (discrete) column 51. data (discrete) column 52. data (discrete) column 53. data (discrete) column 54. data (discrete) column 55. data (discrete) column 56. data (discrete) column 57. data (discrete) column 58. data (discrete) column 59. data (discrete) column 60. data (discrete) column 61. data (discrete) column 62. data (discrete) column 63. data (discrete) column 64. data (discrete) column 65. data (discrete) column 66. data (discrete) column 67. data (discrete) column 68. data (discrete) column 69. data (discrete) column 70. data (discrete) column 71. data (discrete) column 72. data (discrete) 7. Flagging Criteria: column 1. flag_locationcoords(col_Location,col_Longitude,col_Latitude)="Q" column 2. x<-90="I", x>90="I", x<28="Q", x>44="Q", flag_locationcoords(col_Location,col_Longitude,col_Latitude)="Q" column 3. x<-180="I", x>180="I", x<-97="Q", x>-70="Q", flag_locationcoords(col_Location,col_Longitude,col_Latitude)="Q" column 4. x<1="I", x>31="I" column 5. x<1="I", x>12="I" column 6. x<2009="Q", x>2010="Q" column 7. x<0="I", x>1="I" column 8. x<0="I", x>100="I", x>99="Q" column 9. x<0="I", x>100="I", x<1="Q", x>99="Q" column 10. x<0="I", x>100="I", x<1="Q", x>99="Q" column 11. x<0="I", x>100="I", x>10="Q" column 12. x<0.1="I", x>3="I" column 13. x<0="I", x>100="I" column 14. x<0="I", x>100="I" column 15. x<10="I", x>200="I" column 16. x<0="I", x>3000="I" column 17. x<0="I", x>3000="I" column 18. x<0="I", x>1="I" column 19. x<0="I", x>500="I" column 20. x<0="I", x<5="Q", x>30="Q" column 21. x<0="I", x>500="I" column 22. x<0="I", x>500="I" column 23. x<0="I", x>500="I" column 24. x<0="I", x>500="I" column 25. x<0="I", x<0.1="Q", x>3="Q" column 26. x<1="I", x>4="I" column 27. x<0="I", x>1000="Q" column 28. x<0="I", x>1000="Q" column 29. x<0="I", x>1000="Q" column 30. x<0="I", x>1000="Q" column 31. x<0="I", x>1000="Q" column 32. x<0="I", x>1000="Q" column 33. x<0="I", x>1000="Q" column 34. x<0="I", x>1000="Q" column 35. x<0="I", x>1000="Q" column 36. x<0="I", x>1000="Q" column 37. x<0="I", x>1000="Q" column 38. x<0="I", x>1000="Q" column 39. x<0="I", x>1000="Q" column 40. x<0="I", x>1000="Q" column 41. x<0="I", x>2000="Q" column 42. x<0="I", x>2000="Q" column 43. x<0="I", x>2000="Q" column 44. x<0="I", x>2000="Q" column 45. x<0="I", x>2000="Q" column 46. x<0="I", x>4000="Q" column 47. x<0="I", x>2000="Q" column 48. x<0="I", x>2000="Q" column 49. x<0="I", x>2000="Q" column 50. x<0="I", x>2000="Q" column 51. x<0="I", x>2000="Q" column 52. x<0="I", x>2000="Q" column 53. x<0="I", x>2000="Q" column 54. x<0="I", x>2000="Q" column 55. x<0="I", x>2000="Q" column 56. x<0="I", x>2000="Q" column 57. x<0="I", x>2000="Q" column 58. x<0="I", x>2000="Q" column 59. x<0="I", x>2000="Q" column 60. x<0="I", x>2000="Q" column 61. x<0="I", x>2000="Q" column 62. x<0="I", x>2000="Q" column 63. x<0="I", x>2000="Q" column 64. x<0="I", x>2000="Q" column 65. x<0="I", x>2000="Q" column 66. x<0="I", x>2000="Q" column 67. x<0="I", x>2000="Q" column 68. x<0="I", x>2000="Q" column 69. x<0="I", x>2000="Q" column 70. x<0="I", x>3000="Q" column 71. x<0="I", x>2000="Q" column 72. x<0="I", x>2000="Q" C. Data Anomalies: V. Supplemental Descriptors A. Data Acquisition 1. Data Forms: 2. Form Location: 3. Data Entry Validation: B. Quality Assurance/Quality Control Procedures: C. Supplemental Materials: D. Computer Programs: E. Archival Practices: F. Publications: not specified G. History of Data Set Usage 1. Data Request History: not specified 2. Data Set Update History: none 3. Review History: none 4. Questions and Comments from Users: none