Georgia Coastal Ecosystems LTER Data Set Details

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GCE-LTER Data Set Summary

Accession:

MIC-GCED-1503

Research Theme:

Microbiology (Directed Study)

Contributors:

James T. Hollibaugh, Qian Liu, Bradley Tolar, Jen Mou

Title:

Concentrations, turnover rates and fluxes of polyamines in coastal waters of the South Atlantic Bight during April and October 2011

Abstract:

Polyamines are short-chain aliphatic compounds containing multiple amine groups. They are important components of the cytosol of eukaryotes and are present at mmol/L concentrations inside phytoplankton cells, while complex polyamines play a role in biosilica deposition. Concentrations of polyamines measured in seawater are typically in the sub nmol/L range, implying rapid and efficient uptake by osmotrophs, likely bacterioplankton. We measured turnover rates of three polyamines (putrescine, spermidine and spermine) using 3H-labeled compounds and determined their concentrations by HPLC to estimate polyamine contributions to dissolved organic matter and bacterioplankton carbon and nitrogen demand. These measurements were made on transects from the inner shelf to the Gulf Stream across the South Atlantic Bight (SAB) during April and October of 2011 and in salt marsh estuaries on the Georgia coast during August of 2011 and April of 2012. This data set includes measurements of water column variables (temperature, salinity, biogenic Silica), nutrients (nitrite, nitrate+nitrite, ammonium and dissolved inorganic nitrogen), and concentrations and turnover rates of polyamine compounds.

DOI:

10.6073/pasta/1532f1deb5f20dd05715e2704edf6a50

Key Words:

bacteria, dissolved inorganic nitrogen, nitrate, nitrite, nitrogen, polyamines, putrescine, South Atlantic Bight, spermidine, spermine

LTER Core Area:

Inorganic Matter

Research Themes:

Microbiology, General Nutrient Chemistry

Study Period:

18-Apr-2011 to 06-Oct-2011

Study Sites:

GCE-OS -- Off-shore Transect, Georgia, USA

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Publications:

Liu, Q., Lu, X., Tolar, B., Mou, X. and Hollibaugh, J.T. 2015. Concentrations, turnover rates and fluxes of polyamines in coastal waters of the South Atlantic Bight. Biogeochemistry. Online Early. (DOI: 10.1007/s10533-014-0056-1)

Downloads:

Data Table: MIC-GCED-1503 (Main data table for data set MIC-GCED-1503, 51 records)

Access: Public (released 13-Mar-2017)

Metadata: Text (ESA FLED), XML (Ecological Metadata Language)

Data Formats: Spreadsheet (CSV) [16.34kb], Text File [15.58kb], MATLAB (GCE Toolbox) [326.16kb], MATLAB (Variables) [210.08kb], Text Report [56.66kb]

Column List:(hide)

Column	Name	Units	Type	Description
1	Date	YYYY-MM-DD	string	Date of sample collection
2	StationID	none	integer	Numeric station identifier for the cruise transect
3	Station_Name	none	string	Name of the samplnig station
4	Location	none	string	Identifier of the station in the GCE-LTER geographic database
5	Latitude	degrees	floating-point	Geographic latitude
6	Longitude	degrees	floating-point	Georgraphic longitude
7	Depth	m	floating-point	Depth of sample collection as reported by the rosette CTD
8	Temperature	°C	floating-point	Water temperature at depth of sample collection as reported by the rosette CTD
9	Salinity	PSU	floating-point	Salinity at depth of sample collection as reported by the rosette CTD
10	BSi_Conc	micromolar	floating-point	Concentration of biogenic Silica
11	Leucine_Incorp	pM/h	floating-point	Incorporation rate of tritiated Leucine
12	Flag_Leucine_Incorp	none	string	QA/QC flags for Incorporation rate of tritiated Leucine (flagging criteria, where "x" is Leucine_Incorp: x<0="I")
13	Archaea_amoA	copies/L	exponential	Abundance of Archaea ammonia monooxygenase genes per liter of water sampled
14	Crenarchaea_16S	copies/L	exponential	Abundance of Crenarchaea 16S rRNA genes per liter of water sampled
15	Bacteria_amoA	copies/L	exponential	Abundance of Betaproteobacteria ammonia monooxygenase genes per liter of water sampled
16	Bacteria_16S	copies/L	exponential	Abundance of Bacteria genes per liter of water sampled
17	Nitrospina_16S	copies/L	exponential	Abundance of Nitrospina 16S rRNA genes per liter of water sampled
18	Diatom_18S	copies/L	exponential	Abundance of diatom genes per liter of water sampled
19	Nitrite_Conc	micromolar	floating-point	Concentration of nitrite
20	NOx_Conc	micromolar	floating-point	Concentration of nitate plus nitrite
21	NH4_Conc	micromolar	floating-point	Concentration of ammonium
22	DIN_Conc	micromolar	floating-point	Concentration of dissolved inorganic nitrogen
23	Mean_Putrescine_Turnover	1/d	floating-point	Putrescine turnover rate, mean of replicate measurements
24	SE_Putrescine_Turnover	1/d	floating-point	Putrescine turnover rate, standard error of replicate measurements
25	Mean_Spermidine_Turnover	1/d	floating-point	Spermidine turnover rate, mean of replicate measurements
26	SE_Spermidine_Turnover	1/d	floating-point	Spermidine turnover rate, standard error of replicate measurements
27	Mean_Spermine_Turnover	1/d	floating-point	Spermine turnover rate, mean of replicate measurements
28	SE_Spermine_Turnover	1/d	floating-point	Spermine turnover rate, standard error of replicate measurements
29	Mean_Arginine_Turnover	1/d	floating-point	Arginine turnover rate, mean of replicate measurements
30	SE_Arginine_Turnover	1/d	floating-point	Arginine turnover rate, standard error of replicate measurements
31	Mean_GlutamicAcid_Turnover	1/d	floating-point	Glutamic Acid turnover rate, mean of replicate measurements
32	SE_GlutamicAcid_Turnover	1/d	floating-point	Glutamic Acid turnover rate, standard error of replicate measurements
33	Mean_Putrescine_Conc	nanomolar	floating-point	Putrescine concentration, mean of replicate measurements
34	SE_Putrescine_Conc	nanomolar	floating-point	Putrescine concentration, standard error of replicate measurements
35	Mean_Spermidine_Conc	nanomolar	floating-point	Spermidine concentration, mean of replicate measurements
36	SE_Spermidine_Conc	nanomolar	floating-point	Spermidine concentration, standard error of replicate measurements
37	Mean_Spermine_Conc	nanomolar	floating-point	Spermine concentration, mean of replicate measurements
38	SE_Spermine_Conc	nanomolar	floating-point	Spermine concentration, standard error of replicate measurements
39	Mean_Arginine_Conc	nanomolar	floating-point	Arginine concentration, mean of replicate measurements
40	SE_Arginine_Conc	nanomolar	floating-point	Arginine concentration, standard error of replicate measurements
41	Mean_GlutamicAcid_Conc	nanomolar	floating-point	Glutamic Acid concentration, mean of replicate measurements
42	SE_GlutamicAcid_Conc	nanomolar	floating-point	Glutamic Acid concentration, standard error of replicate measurements

Statistics:

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Citation:

Hollibaugh, James T. 2015. Concentrations, turnover rates and fluxes of polyamines in coastal waters of the South Atlantic Bight during April and October 2011. Georgia Coastal Ecosystems LTER Project, University of Georgia, Long Term Ecological Research Network. http://dx.doi.org/10.6073/pasta/1532f1deb5f20dd05715e2704edf6a50

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