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GCE-LTER Data Set Summary

Accession: BCT-GCEM-0511e Research Theme: Bacterial Productivity (Monitoring)
Contributors: Robert E. Hodson, Jen Mou, Ed Sheppard
Title: May 2004 surface water bacterial abundance at ten Georgia Coastal Ecosystems LTER sampling sites
Abstract: Surface water samples were collected during low tide surveys near ten Georgia Coastal Ecosystem LTER sampling sites in May, 2004. Unfiltered subsamples were immediately preserved with buffered formalin and refrigerated for transport to the laboratory. The abundance of bacteria in each sample was determined using an epifluorescence microscope by counting DAPI-stained cells retained on a 0.2µm polycarbonate filter. This study was part of the GCE-LTER hydrographic monitoring program, and will be repeated quarterly.
DOI: 10.6073/pasta/637e2cd23dc11813e1c4dcf338bfaa3a
Key Words: abundance, bacteria, bacterial abundance, bacterial production, microbial, Microbial Populations, water column
LTER Core Area: Population Studies
Research Themes: Bacterial Productivity
Study Period: 28-May-2004 to 30-May-2004
Study Sites:
GCE1 -- Eulonia, Georgia, USA
GCE2 -- Four Mile Island, Georgia, USA
GCE3 -- North Sapelo, Sapelo Island, Georgia, USA
GCE4 -- Meridian, Georgia, USA
GCE5 -- Folly River, Georgia, USA
GCE6 -- Dean Creek, Sapelo Island, Georgia, USA
GCE7 -- Carrs Island, Georgia, USA
GCE8 -- Alligator Creek, Georgia, USA
GCE9 -- Rockdedundy Island, Georgia, USA
GCE10 -- Hunt Camp, Sapelo Island, Georgia, USA
GCE-SP -- Sapelo River, Georgia, USA
GCE-DB -- Doboy Sound, Georgia, USA
GCE-AL -- Altamaha River, Georgia, USA
GCE-DP -- Duplin River, Georgia, USA
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Data Table: BCT-GCEM-0511e (Main data table for data set BCT-GCEM-0511e, 30 records)

Access: Public (released 14-Nov-2005)

Metadata: Text (ESA FLED), XML (Ecological Metadata Language)

Data Formats: Spreadsheet (CSV) [5.30kb],  Text File [3.72kb],  MATLAB (GCE Toolbox) [262.84kb],  MATLAB (Variables) [167.21kb],  Text Report [35.94kb]

Column List:(hide)

Column Name Units Type Description
1 Cruise none string GCE-LTER research cruise identifier (LTER[MM][YY])
2 Date_Sampled yyyy-mm-dd string Date sample was collected
3 Time_Sampled hh:mm GMT string Time sample was collected (GMT)
4 Latitude decimal degrees floating-point Geographic latitude of sampling location
5 Longitude decimal degrees floating-point Geographic longitude of sampling location
6 Station none string Nearest nominal GCE-LTER CTD profiling station (1km geo-reference tolerance for station matching)
7 Site none integer Closest nominal GCE sampling site
8 Vessel none string Vessel from which the sample was collected
9 CTD_Cast none string CTD cast number corresponding to the sample (na = not applicable)
10 Depth_Sampled none string Nominal sampling depth
11 Temperature °C floating-point Water temperature at the sampling location
12 Flag_Temperature none string QA/QC flags for Water temperature at the sampling location (flagging criteria, where "x" is Temperature: x<10="Q", x>35="Q", x<-2="I")
13 Salinity PSU floating-point Salinity of the water at the sampling location
14 Flag_Salinity none string QA/QC flags for Salinity of the water at the sampling location (flagging criteria, where "x" is Salinity: x>36="Q", x<0="I")
15 Tide none string Nominal tide stage when the sample was collected
16 Volume ml floating-point Sample volume filtered
17 Flag_Volume none string QA/QC flags for Sample volume filtered (flagging criteria, where "x" is Volume: x<0="I", x<0.1="Q", x>10="Q")
18 Tower_Diameter mm floating-point Filter tower diameter
19 Flag_Tower_Diameter none string QA/QC flags for Filter tower diameter (flagging criteria, where "x" is Tower_Diameter: x<0="I")
20 Mean_Bact_Per_Field cells/field floating-point Mean bacteria per field for 10 microscope fields
21 Flag_Mean_Bact_Per_Field none string QA/QC flags for Mean bacteria per field for 10 microscope fields (flagging criteria, where "x" is Mean_Bact_Per_Field: x<5="Q", x>300="Q", x<0="I")
22 SD_Bact_Per_Field cells/field floating-point Standard deviation of replicate counts
23 Flag_SD_Bact_Per_Field none string QA/QC flags for Standard deviation of replicate counts (flagging criteria, where "x" is SD_Bact_Per_Field: x<0="I")
24 Bact_Abundance cells/ml exponential Bacterial abundance calculated from direct count and volume filtered
25 Flag_Bact_Abundance none string QA/QC flags for Bacterial abundance calculated from direct count and volume filtered (flagging criteria, where "x" is Bact_Abundance: x<1e4="Q", x>1e7="Q", x<0="I", SD_Bact_Per_Field>(Mean_Bact_Per_Field).*0.5="Q")
Statistics: Generate script code to retrieve data tables for analysis in: MATLAB, R, SAS, SPSS
Citation: Hodson, Robert E. 2005. May 2004 surface water bacterial abundance at ten Georgia Coastal Ecosystems LTER sampling sites. Georgia Coastal Ecosystems LTER Project, University of Georgia, Long Term Ecological Research Network. http://dx.doi.org/10.6073/pasta/637e2cd23dc11813e1c4dcf338bfaa3a

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This material is based upon work supported by the National Science Foundation under grants OCE-9982133, OCE-0620959, OCE-1237140 and OCE-1832178. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.